快速篩選有機磷農藥之光學生物感測器應用

Application of Optical Biosensor for Rapid Screen of Organophosphorus Pesticides

何唐興(Tang-Hsing Ho)

指導教授 ： 董瑞安

國立清華大學/原子科學院/生醫工程與環境科學系/碩士(2008年)

摘要

台灣地狹人稠且處於高溫多溼的亞熱帶氣候區，易於遭受病蟲的侵害，為了確保農作物生產的數量及穩定性，勢必須對易於遭受病蟲害及具有高經濟價值之農作物噴灑大量的農藥，因而產生農藥使用不當而危害一般消費者之狀況。有鑑於此，蔬果中之農藥殘留量檢測成為食品檢測中重要的一環。一般檢驗蔬果農藥殘留量須使用氣相或液相層析法配合適當的偵測器及前處理始可測得。缺乏一個兼具靈敏、簡單、方便、快速且能同時量測多種農藥之檢測方法，便於一般非專業分析人員使用。因此，為達成上述目的，多數文獻著重於利用農藥對酯解□之抑制作用研發生物感測器，以期具有靈敏、快速且能同時偵測多種農藥之功能。
　本研究的目的在於應用農藥對酵素的抑制作用及Ellman之光學檢測方法，建立出一個可靈敏且快速篩選多種農藥殘量之生物感測器。由研究結果顯示，此感測器在15分鐘內即可偵測完成，且可測得多種常用之有機磷及氨基甲酸酯類農藥如加保扶及陶斯松等，偵測極限值為0.5及0.025 ppm，低於農藥殘存濃度限量值，可有效篩選高於管制殘存量之樣品，且均有一個數量級以上之偵測範圍。此外，根據長時間保存及實際樣品之實驗結果，本感測器維持相同之抑制值達四星期以上，且可用於量測實際樣品。

關鍵字

有機磷農藥 ； 生物感測器 ； 乙醯膽鹼酯解□ ； Ellman方法 ； 酵素抑制

並列摘要

Taiwan locates in tropic and subtropic areas with high temperatures and humidity. The breeding of insects for crops is easily happened at high temperature and humid areas. It is important for farmers in the high density farm to use high dosages of pesticides. The detection of pesticide residues in foods usually need expensive instruments such as gas chromatography or high performance liquid chromatography. Therefore, it is necessary to develop a small, cheap, easily to handle and able to provide reliable information biosensor that can be used in real-time and in situ analysis.
In this study, we developed a AChE-based biosensor with fluid channel using the Ellman’s reagents. Results show that three pesticides: profenofos, carbofuran, and chlorpyrifos have different sensitivity toward AChE biosensors. The limit of detection were 200, 0.5, 0.025 ppm respectively . The analytical range were large than one order of magnitude for these pesticides. Furthermore, the results obtained from biosensor method were compared with the Rapid Bioassay for Pesticide Residues(RBPR) to understand the analytical performance of the biosensors. The performance of this biosensor was similar to RBPR. In addition, results of long-term stability show that the inhibition response has no obviously change after 4 week of storage.

並列關鍵字

Organophosphorus Pesticides ； biosensor ； Acetylcholinesterase ； Ellman’s method ； Enzyme inhibition

參考文獻

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