以破囊壺菌Aurantiochytrium limacinum SR21醱酵生產二十二碳六烯酸(DHA)之研究

Production of docosahexaenoic acid (DHA) by Aurantiochytrium limacium SR21

黃丁晏

二十二碳六烯酸 ； 破囊壺菌 ； 甘油 ； 油酸 ； 脂肪酸 ； Docosahexaenoic acid ； DHA ； Glycerol ； Aurantiochytrium limacinum SR21 ； Total fatty acid ； Oleic Acid

清華大學化學工程學系學位論文

2013年

博士

朱一民

繁體中文

近年來，Aurantiochytrium limacinum SR21因具高油脂含量與高docosahexaenoic acid (DHA, C22:6) 產量而受到重視。本研究利用間歇性饋氧方式以維持溶氧平均值於50 %，並產生一溶氧震盪的醱酵環境，促使Aurantiochytrium limacinum SR21同時進行生長與油脂累積生成。實驗結果發現，碳氮比1.25與饋料濃度為Glycerol 100 g L-1、Yeast extract 40 g L-1, Peptone 40 g L-1的情況下，Aurantiochytrium limacinum SR21最高菌體濃度可達61.76 g L-1，油脂含量最高65.2%、DHA濃度20.3 g L-1、DHA產率122.62 mg L-1 h-1。溶氧震盪的醱酵環境可以改變Aurantiochytrium limacinum SR21SR21胞內脂肪酸組成。在醱酵中後期，Aurantiochytrium limacinum SR21胞內的飽和脂肪酸C16:0比例大幅降低至5 %以下，並提高不飽和脂肪酸C22:6的比例至70 %。 藉由添加Oleic Acid並結合溶氧震盪的醱酵環境，可促使Aurantiochytrium limacinum SR21將油酸轉化合成DHA。實驗結果發現，以Oleic Acid 50 g L-1進行醱酵培養，Aurantiochytrium limacinum SR21可獲得最高菌體濃度可達44.87 g L-1，油脂含量最高95.4 %、DHA濃度23.54 g L-1、DHA產率367.79 mg L-1 h-1。此實驗結果可以大幅降低DHA原料成本，且大幅提升DHA產率。

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