本研究採用無乳化聚合法合成表面帶不同電荷的聚苯乙烯,與蛋白質在不同的pH值下進行吸附之研究;利用陽離子起始劑(AIBA)成功的合成出表面帶正電荷之均一粒徑聚苯乙烯,平均粒徑大小為500nm,表面電荷約為+19.7mv;而利用陰離子起始劑(K2S2O8)成功的合成出表面帶負電荷之均一粒徑聚苯乙烯,平均粒徑大小為586nm,表面電荷約為-23.96mv。 將表面帶不同電荷的粒子與蛋白質進行吸附作用,(在pH>pI值下蛋白質表面帶負電荷,在pH<pI值下蛋白質表面帶正電荷),發現在pH=3時BSA帶正電與表面帶負電荷的材料有很強的吸收;相反的當pH=6時BSA帶負電與表面帶正電荷的材料有較強的吸收效果。 研究第二部份仍是利用無乳化聚合方法,將苯乙烯(styrene)與甲基丙烯酸環氧丙酯(GMA)進行共聚合作用,利用陽離子當起始劑(AIBA),成功的合成出表面帶正電荷之均一粒徑P(S-co-GMA),平均粒徑大小為205nm,表面電荷約為+30.27mv;利用P(S-co-GMA)在材料表面能提供環氧基的特性,進行聚合物表面改質,使粒子表面接上不同官能基,利用改質的顆粒與蛋白質進行吸附作用,發現表面有改質的顆粒比聚苯乙烯粒子有更好的吸附效果,推測蛋白質與顆粒進行吸附時P(S-co-GMA)的顆粒較小,表面積增大,加強吸附的效果,所以只需要10mg即可將50μg/ml的BSA完全吸附起來,比未改質的聚苯乙烯粒子吸附效果更好,提供一個很好的研究方向。
The emulsion-free emulsion polymerization technique is used to synthesize the polystyrene that carries different surface voltage. The charge then polystyrene can adsorb BSA protein by electrostatic force under different pH values. The initiator used during the synthesis is the factor to determine the different surface voltages of polystyrene product. When the AIBA initiator is used,the polystyrene particles with diameters averaging 500nm and +19.70mv surface charge are obtained. While the KPS initiator,the polystyrene particles with diameters averaging 586nm and –23.96mv are obtained. The polystyrene particles are tested for their ability to attract to proteins under different pH values (when pH > pI,the protein carries a negative charge and when pH < pI ,the protein carries a positive charge). It is found that when pH = 3 BSA carries a positive charge and thus have stronger affinity for negative charged polystyrene particles. Whereas at pH = 6 BSA carries a negative charge and thus have stronger affinity for positively charged polystyrene particles. In the second part of this study,styrene and GMA underwent co polymerization by AIBA as initiator . The copolymers of P(S-co-GMA) have diameter averaging 205nm and the surface voltage is about +30.27mV. About 10mg of the modified P(S-co-GMA) surface is enough to completely absorb 50μg/ml of BSA . It is better than unrefined polystyrene. It is speculated since the P(S-co-GMA) has a smaller particle size (thus bigger surface area per gram sample) as well as the refinement of the surface. This gives a good start for further research on this area.
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