毛細力牽引試劑晶片之製作

Study of chip fabrication on dragging reagent by capillary force

林育德(Yu-De Lin)

指導教授 ： 張耀仁

中原大學/工學院/機械工程研究所/碩士(2016年)

https://doi.org/10.6840/cycu201600071

摘要

微型幫浦在微流道晶片上通常做為驅動力注入和推動試劑。在本研究中，提出了一個新型的毛細閥設計。這種引導式毛細閥具有倒角邊能保持注入液體在閥的地方。由於微通道的表面被改質為親水性，注入到主通道中的試劑由於試劑的毛細力可以自然流動。不需要任何外部設備來驅動試劑。當試劑流經引導毛細閥，被保存的試劑可以牽引出儲存槽進行進一步的反應。為了測試毛細閥的性能，黃色染料和紅色染料溶液被用作於測試的材料。相較於T型毛細管閥（沒有倒角側），該引導毛細閥具有更好的性能。逆流現象，無論是從儲存槽至主流道，反之亦然，容易在T型毛細閥發生。此外，被牽引的紅色染料溶液會阻礙黃色染料溶液流入主流道。黃色染料溶液與紅色染料溶液的比率為1：2，導致不等量為反應液。相反地，這個引導毛細閥改善了逆流的現象。在儲存槽與主要液體槽中沒有觀察到逆流現象。而牽引的效果使得黃色和紅色染料溶液的比例為1：1。在此研究中，此引導式的晶片也被應用在血型試驗。 4μL的3％紅血球細胞（RBC）注入到主流道分別牽引試劑，如抗體、低離子介質（LIM）、0.05％聚凝胺和重懸溶液。混合後，凝集的結果是通過測量穿透光束的功率變化。便能獲得準確的血液分析測試結果。

關鍵字

毛細力 ； 微流道

並列摘要

Micro-pumps are usually used as the driving force to inject and to propel the reagents in the microchannel chip. In this study, a chip with a novel design of capillary valve is presented. This proposed capillary valve has a chamfered side to hold the injected liquid in valve position. Since the surface of the microchannels is modified to be hydrophilic, the reagent injected into the main channel can flow naturally due to the reagent’s capillary force. No external devices are required to drive the reagent. When the reagent flows through the proposed capillary valve, the kept liquid can be dragged out of the chamber for further reaction. To examine the performance of the capillary valve, yellow dye and red dye solutions were used as the materials for the test. Compared with the T-type capillary valve (without the chamfered side), this proposed capillary valve has better performance. The countercurrent phenomena, either from the storage chamber to the main channel or vice versa, are prone to occur for the T-type capillary valve. Moreover, the dragged red dye solution blocks the flow path of yellow dye solution injected into the main channel. The ratio of yellow dye solution to red dye solution was 1:2, resulting in unequal quantities of solutions for reaction. On the contrary, this proposed capillary valve improves the countercurrent phenomena. No countercurrents were observed in the storage chamber and injection chamber. The dragging effect allowed the ratio of yellow and red dye solutions to be 1:1. This proposed chip was also implemented to the blood typing tests in this study. Four microliter of 3% red blood cells (RBC) was injected into the main channel to drag the reagents, such as antibody, low ionic medium (LIM), 0.05% polybrene, and resuspending solution, respectively. After mixing, aggregation results were observed by measuring the power of penetrating light beam. Accurate results of blood typing tests were obtained.

並列關鍵字

capillary force ； microfluidics

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