電磁場對小鼠囊胚細胞凋亡與發育之影響

本實驗研究電磁場引起小鼠囊胚期胚胎細胞的凋亡和降低細胞增生的能力，並探討電磁場對胚胎囊胚期後續發育所造成的影響。實驗中取懷孕第四天小鼠子宮中的囊胚期胚胎，分別暴露於0.5及1.0 高斯電磁場中培養二十四小時後，以區別染色觀察囊胚細胞數明顯的發現，分別處理0.5及1.0 高斯電磁場的平均囊胚細胞數低於未處理電磁場的平均囊胚細胞數。而以TUNEL染色法分析囊胚細胞凋亡的情形，結果發現處理1.0 高斯電磁場的囊胚細胞凋亡明顯高於未處理電磁場的囊胚細胞，且以PI 及Annexin-V染色來辨別細胞死亡或凋亡的實驗中，發現分別處理1.0 高斯電磁場的所有囊胚細胞出現細胞凋亡的比例明顯高於未處理電磁場的囊胚細胞。再者囊胚暴露電磁場二十四小時後觀察體外發育的情形，結果發現處理1.0 高斯電磁場與未處理電磁場的囊胚比較，處理電磁場囊胚在培養盤貼附的面積較小且外部型態上較不完整。在動物實驗中，懷孕第一天的小鼠暴露在1.0高斯電磁場七十二小時後取子宮中的囊胚期胚胎觀察，以TUNEL染色法分析雖未發現暴露於電磁場的小鼠囊胚有明顯的細胞凋亡現象，但暴露於電磁場後其含有正常囊胚期胚胎的比例明顯的下降。綜合以上實驗的結果，我們明確的指出1.0 高斯電磁場使得胚胎的細胞數下降及誘導胚胎細胞凋亡，且阻礙囊胚的發育，在動物實驗中也證實了電磁場降低了正常囊胚的比例。這項新穎的發現提供了重要的概念在電磁場對於小鼠的囊胚的影響。

關鍵字

細胞凋亡；電磁場；小鼠囊胚

並列摘要

In this report, we examine the cytotoxic effect of electromagnetic field (EMF) on the blastocyst stage of mouse embryos and on subsequent early postimplantation embryonic development in vitro. In Differential staining revealed that blastocysts treated with 0.5、1.0 G electromagnetic field for 24 h showed a significant reduction in the average number of total cells versus controls. This could be correlated with the observation in terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay and Annexin-V techniques revealed that blastocysts treated with electromagnetic field showed increased apoptosis versus untreated controls. In addition, 1.0 G electromagnetic field-treated blastocyst showed normal levels of implantation on culture dishes in vitro, but significantly fewer hatching area were measured at relatively early stages of development. We examine the cytotoxic effect of electromagnetic field on the mouse embryos in vivo. The TUNEL assay revealed that blastocysts after treated with 1.0 G electromagnetic field for 96 h did not show increased apoptosis, but the proportion of taking all embryos of blastocysts reduced versus untreated controls In conclusion, our results collectively indicate that electromagnetic field treatment of mouse blastocysts induces apoptosis, decreases cell numbers, and retards early postimplantation blastocyst development. These novel findings provide important new insights into the effect of EMF on mouse blastocysts.

並列關鍵字

apoptosis ； mouse blastocyst ； electromagnetic field

參考文獻

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電磁場對小鼠囊胚細胞凋亡與發育之影響

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