二氧化矽螢光奈米管製備與其在基因傳輸上的應用

Preparation of Fluorescent Silica Nanotubes and their Application in Gene Delivery

劉耀中(Yao-Chung Liu)

指導教授 ： 陳家俊

國立臺灣師範大學/理學院/化學系/碩士(2003年)

摘要

二氧化矽奈米管具有特殊中空結構和表面容易修飾等優點，由模版方法可以大量合成材料且有效控制材料的大小。我們利用多孔性模版-陽極氧化鋁（AAO），和二氧化矽（SiO2）的溶膠-凝膠溶液來合成二氧化矽奈米管，再進一步將奈米管和水溶性CdSe(ZnS)奈米粒子結合，形成二氧化矽螢光奈米管。二氧化矽螢光奈米管，可方便我們觀察材料是否進入細胞，以及進入細胞後其所在的位置。將綠色螢光蛋白（GFP）的DNA經由二氧化矽奈米管帶進細胞內，藉由細胞螢光蛋白的表現來證明二氧化矽奈米管在基因傳輸上的應用。

關鍵字

二氧化矽螢光奈米管 ； 基因傳輸

並列摘要

The silica nanotubes have a number of advantages that make them potential candidate for biological applications. Firstly, nanotubes have inner voids that can be filled with species ranging in size from large proteins to small molecules. Secondly, nanotubes have distinct inner and outer surfaces that can be functionalized easily. By using template method, a large number of nanotubes were synthesized and their sizes were controlled preciously. Silica nanotubes were synthesized through a sol-gel reaction using the anodic aluminum oxide membrane (AAO) as a template. The fluorescent silica nanotubes were prepared by dopping water-soluble nanocrystal CdSe(ZnS). The fluorescent silica nanotubes were used to monitor the localization of nanotubes in living cells and found to be localized in the cytoplasm. The fluorescent silica nanotubes carrying the GFP gene were employed to investigate the potential application of the nanotubes in gene delivery. The expression of GFP demonstrates a new biological application of nanotubes as a biomolecule carrier.

並列關鍵字

Fluorescent Silica Nanotube ； Gene delivery

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