Title

直接大氣壓下化學游離質譜法應用於蜂蜜不同蜜源及產地之鑑定

Translated Titles

Identification of Botanical and Geographic Origins of Honey Using Direct APCI-MS Analysis

Authors

吳宗元

Key Words

氣盾式大氣壓化學游離法 ; 直接分析 ; 揮發性 ; 蜂蜜 ; 主成分分析 ; Atmospheric Pressure chemical ionization ; Direct analysis ; Volatiles ; Honey ; PCA

PublicationName

虎尾科技大學生物科技研究所學位論文

Volume or Term/Year and Month of Publication

2015年

Academic Degree Category

碩士

Advisor

沈振峯

Content Language

繁體中文

Chinese Abstract

蜂蜜,主要為蜜蜂採集各種植物之蜜汁或是植物外泌液所釀製而成的天然甜味物質。本實驗利用我們所開發的氣盾式大氣壓下化學游離介面 (Gas-shield atmospheric pressure chemical ionization, GS-AP) 結合大氣壓下質譜儀來分析蜂蜜中揮發性物質的成份。我們以純水將蜂蜜稀釋十倍再以氮氣流將氣味成分送入介面分析以獲得不同蜂蜜樣品之指紋質譜,並將所得到的結果建立指紋圖譜資料庫。結果顯示利用本研究建立之「稀釋/吹氣/GS-AP/MS」分析平台配合蜂蜜氣味質譜資料庫比對可以對於不同蜜源鑑別,在產地鑑別分析,我們使用三種不同分析方法(資料庫搜索、主成分分析、特徵離子對比例)來做為台灣與泰國龍眼蜂蜜鑑定,我們以30個龍眼蜂蜜盲樣樣品進行分析,其三種分析方法正確率為: 資料庫搜索正確率為28/30,主成分分析正確率為25/30,特徵離子對比例正確率為29/30。由結果顯示GS-AP直接分析確實可以鑑別蜂蜜中產地。 關鍵詞: 氣盾式大氣壓化學游離法、直接分析、揮發性、蜂蜜、主成分分析

English Abstract

Honey is a sweet natural product produced by Apis mellifera from the nectar of plants (nectar, honey). Each honey possesses its own aroma and flavor, depending mainly on the botanical sources that contribute to its production. In this study, a simple diret APCI-MS analysis was applied to detect the fingerprinting mass spectra of the honey samlples after 10-fold dilution with water. The obtained mass spectra were found to be useful in the identification of the honey from different botanical and geographic origins. By using the characteristic ions/ion ratios, the mass spectra database (fingerprinting mapping) and PCA strategies, all the idetifications were valid. 30 blind samples were examined with the strategies, the successful ID rates were 29/30 using ion ratio, 28/30 using liberary search and 28/30 using PCA method. The results evidenced the ultilty of the direct APCI-MS analysis for the identification of the honey oringins. Keywords: Atmospheric Pressure chemical ionization, Direct analysis, Volatiles, Honey, PCA

Topic Category 文理學院 > 生物科技研究所
生物農學 > 生物科學
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