We investigate the effect of silver nanostructures on fluorescent emission from Cy3-tagged molecules. We measured the fluorescent intensity and the fluorescent life time of Cy3 molecules deposited on arrays of silver nanostructure. We found that the fluorescent intensity was enhanced by nanostructures and the life time was decreased. The minimum of life time occurred to the nanostructure where the fluorescent intensity is the maximum. We also varied the incident power and characterize the fluorescent contrast between the nanostructures and the flat Ag film. We observed that the contrast decreased with incident power. We also calculate the near-field enhancement, absorption spectrum and reflection spectrum simulation result for all kind of periodical nanostructures. In final, we compare the result of simulation and experiment to find some relation between them. Key word:Fluorescence、Enhancement of Fluorescence