根據國內外研究指出室內的灰塵中含許多內分泌干擾物質(Endocrine disrupter compounds, EDCs),其中包括戴奧辛與類戴奧辛化合物(Dioxin and Dioxin-like compounds)與雌激素與類雌激素化合物(Estrogen and Estrogen-like compounds)。目前法規主要使用化學法HRGC/HRMS偵測EDCs濃度,且該方法具有高敏感度與準確性,但分析需花費大量成本。因此近年來許多學者開始使用生物報導基因法調查EDCs濃度,且reporter gene assay具有良好之敏感度,因此本研究使用Ad-DR bioassay與T47D-KBluc assay調查南部地區20間國小與幼兒園教室地板灰塵中戴奧辛與雌激素毒性當量及其健康風險並探討其潛在污染來源。樣品索式萃取驟參考環保署公佈NIEA S103.61C方法進行。結果顯示,戴奧辛毒性當量範圍介於0.798-16.3(mean:5.14) ng BEQ /kg d.w.,雌激素毒性當量範圍從
Several studies reported that many endocrine disrupter compounds (EDCs) including dioxin and dioxin-like compounds and estrogen and estrogen-like compounds occur mainly in the indoor dust. Chemical analysis (HRGC/HRMS) is the major method for the investigation of EDCs levels in environmental samples. Although chemical analysis has high sensitivity and accuracy, it is mainly costly and was time-consuming. Therefore, the reporter gene assay was established and widely used to survey EDCs levels in the recent year. The aim of this study is to use two reporter gene assays (Ad-DR bioassay and T47D-KBluc assay) to investigate the dioxin toxic equivalency (TEQ) and estrogenic toxic equivalency (EEQ) in twenty elementary schools and kindergartens floor dusts from southern Taiwan, and evaluate the health risk and potential sources. The Soxhlet extraction and cleanup procedures were used in the present study which followed Taiwan Environmental Protection Administration (EPA) standard method (NIEA S103.61C). In this study, the concentration of TEQs and EEQs are ranged 0.798-16.3 (mean:5.14) ng BEQ /kg d.w. and