Title

溪流中雄性尼羅口孵魚之卵黃前質基因表現與內分泌干擾物質之流佈探討

Translated Titles

The distribution of endocrine disrupting chemicals and vitellogenin expression of male Oreochromis niloticus in river

DOI

10.6346/NPUST.2014.00039

Authors

郭旭芩

Key Words

尼羅口孵魚 ; 卵黃前質 ; 內分泌干擾物質 ; 雌激素類化合物 ; 烷基酚類 ; Oreochromis niloticus ; Vitellogenin ; Endocrine disruptors ; Estrogenic Compounds ; Alkylphenols

PublicationName

屏東科技大學環境工程與科學系所學位論文

Volume or Term/Year and Month of Publication

2014年

Academic Degree Category

碩士

Advisor

謝季吟

Content Language

繁體中文

Chinese Abstract

許多研究指出內分泌干擾物質常分佈於河流環境中。然而,鮮少提及有關台灣河流中雌激素化學物質會誘導出雄魚體之卵黃前質 (VTG)之研究。本研究目的為探討內分泌干擾化學物質之濃度:從台灣南部溪流進行污水處理廠的上游(A站)、污水處理廠放流水 (B站),從污水處理廠放流水與溪流交匯後100公尺處(C站)及污水處理廠下游 (D站)中之雌酮 (E1)、雌二醇(E2)、雌三醇(E3)、乙炔基雌二醇(EE2)、己烯雌酚(DES)、壬基苯酚(NP)、辛基苯酚(OP)及雙酚A (BPA)的濃度測定。此外,也利用反轉錄聚合酶鏈反應 (RT-PCR)進行溪流中雄性尼羅口孵魚之VTG表現測定。研究結果顯示,從污水處理廠上游至下游四個測站中發現雄性尼羅口孵魚VTG呈陽性反應分別為26% (n=31)、0% (n=21)、6% (n=17)及50% (n=22)。污水處理廠上游及下游處之兩測站水體樣品的平均濃度分別為5.04 ± 3.79與4.60 ± 1.87 (E1)、41.3 ± 49.3與18.7 ± 29.1 (NP)、3.22 ± 1.30與7.83 ± 10.3 (OP)、5.04 ± 3.79與12.9 ± 24.2 (BPA) ng/L。推論溪流可能接受到工業廢水或當地蔬果市場之污水的排放,使得此兩站被檢出較多化合物濃度。B站僅檢出NP及BPA兩種化合物,其平均濃度分別為7.05 ± 10.3與3.88 ± 2.47 ng/L。C站也同樣檢測出26.1 ± 65.4 (NP)及4.53 ±3.00 (BPA)之濃度,另外還檢出4.04 ± 2.90 (OP) ng/L。本研究在四個測站的水體均未檢測出雌二醇(E2)、雌三醇(E3)、乙炔基雌二醇 (EE2)、己烯雌酚(DES)之濃度。 本研究結果證實,在溪流中檢出率高的內分泌干擾物質與採集之雄魚卵黃前質呈陽性反應有類似趨勢。結果顯示當魚體曝露在水體低濃度烷基酚類混合化學物質可能為相對之雌激素活性之來源。因此,常見物種的卵黃前質生成可以成為評斷環境中內分泌干擾物質效應的生物標記。

English Abstract

The distributions of endocrine disruptors were conducted in the riverine environment in many reports. However, little is known about the induction of vitellogenin in male fish by estrogenic chemicals in Taiwan’s river. This study aimed to determine the concentrations of endocrine disrupting chemicals: estrone(E1), 17β-estradiol (E2), estriol (E3), 17α-ethinylestradiol (EE2), diethylstilbestrol (DES), nonylphenol (NP), octylphenol (OP) and bisphenol-A (BPA) in upstream of wastewater treatment plant (site A), wastewater treatment plant effluent (site B), 0.1 km from the outfall of the wastewater treatment plant (site C) and downstream of the wastewater treatment plant (site D) of the river in Southern Taiwan. In addition, vitellogenin expression of the male Oreochromis niloticus using reverse transcription-polymerase chain reaction (RT-PCR) system have also investigated. The result indicated the elevated VTG response shown in the four sampling sites along the river were 26% (n=31), 0% (n=21), 6% (n=17) and 50% (n=22) from upstream to downstream, respectively. The average concentrations in samples collected, from sites upstream and downstream of WWTP, were 5.04 ± 3.79 and 4.60 ± 1.87 (E1), 41.3±49.3 and 18.7±29.1 (NP), 3.22±1.30 and 7.83 ± 10.3 (OP) and 5.04±3.79 and 12.9 ± 24.2 (BPA) ng/L, respectively. More compounds were detected than the other two sampling sites may related to the industrial and locally farmers’ markets wastewater discharged. Only two of the eight compounds, NP and BPA, were detected in site B and the average concentrations were 7.05 ± 10.3 and 3.88 ± 2.47 ng/L. These compounds were also detected in site C with the concentrations of 26.1± 65.4(NP), and 4.53±3.00 (BPA) and in addition, 4.04±2.90 (OP) ng/L. Compounds 17β-estradiol (E2), 17α-ethinylestradiol (EE2), estriol(E3), diethylstilbestrol (DES) were not detected from samples at various sampling sites during the sampling events. Our results demonstrated a parallel pattern that higher detecting frequency of the endocrine disruptors to the VTG production in male fish collected. The results indicated the possibility of that these chemicals probably account for the estrogenic activity in this river while the fish exposed in the low concentrations of alkylphenolic mixture. Therefore, the vitellogenesis of common species can be a useful biomarker to evaluate the impact of endocrine disrupting chemicals in the environment.

Topic Category 工學院 > 環境工程與科學系所
工程學 > 土木與建築工程
Reference
  1. Abdel-Tawwab, M., M. A. Mousa, M. H. Ahmad and S. F. Sakr, 2007, “The use of calcium pre-exposure as a protective agent against environmental copper toxicity for juvenile Nile tilapia, Oreochromis niloticus,” Aquaculture, Vol.264, No.1, pp.236-246.
    連結:
  2. Ademollo, N., F. Ferrara, M. Delise, F. Fabietti and E. Funari, 2008, “Nonylphenol and octylphenol in human breast milk,” Environment International, Vol.34, No. 7, pp.984-987.
    連結:
  3. Aerni, H.-R., B. Kobler, B. V. Rutishauser, F. E. Wettstein, R. Fischer, W. Giger, A. Hungerbühler, M. D. Marazuela, A. Peter and R. Schönenberger, 2004, “Combined biological and chemical assessment of estrogenic activities in wastewater treatment plant effluents,” Analytical and Bioanalytical Chemistry, Vol.378, No.3, pp. 688-696.
    連結:
  4. Amano, H., T. Fujita, N. Hiramatsu, H. Kagawa, T. Matsubara, C. V. Sullivan and A. Hara, 2008, “Multiple vitellogenin‐derived yolk proteins in gray mullet (Mugil cephalus): Disparate proteolytic patterns associated with ovarian follicle maturation,” Molecular Reproduction and Development, Vol.75, No.6, pp.1307-1317.
    連結:
  5. Amano, H., T. Fujita, N. Hiramatsu, M. Shimizu, S. Sawaguchi, T. Matsubara, H. Kagawa, M. Nagae, C. V. Sullivan and A. Hara, 2007, “Egg yolk proteins in grey mullet (Mugil cephalus): purification and classification of multiple lipovitellins and other vitellogenin‐derived yolk proteins and molecular cloning of the parent vitellogenin genes,” Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, Vol.307, No.6, pp.324-341.
    連結:
  6. Arcand-Hoy, L., A. Nimrod and W. Benson, 1998, “Endocrine-modulating substances in the environment: estrogenic effects of pharmaceutical products,” International Journal of Toxicology, Vol.17, No.2, pp.139-158.
    連結:
  7. Arditsoglou, A. and D. Voutsa, 2010, “Partitioning of endocrine disrupting compounds in inland waters and wastewaters discharged into the coastal area of Thessaloniki, Northern Greece,” Environmental Science and Pollution Research, Vol.17, No.3, pp.529-538.
    連結:
  8. Arnold, S. F., P. Vonier, B. M. Collins, D. M. Klotz, L. J. Guillette Jr and J. A. McLachlan, 1997, “In vitro synergistic interaction of alligator and human estrogen receptors with combinations of environmental chemicals,” Environmental Health Perspectives, Vol.105, No.3, pp.615.
    連結:
  9. Arukwe, A., 2001, “Cellular and molecular responses to endocrine-modulators and the impact on fish reproduction,” Marine Pollution Bulletin, Vol.42, No.8, pp. 643-655.
    連結:
  10. Bjerregaard, L. B., C. Lindholst, B. Korsgaard and P. Bjerregaard, 2008a, “Sex hormone concentrations and gonad histology in brown trout (Salmo trutta) exposed to 17β-estradiol and bisphenol A,” Ecotoxicology, Vol.17, No.4, pp.252-263.
    連結:
  11. Bjerregaard, P., P. R. Hansen, K. J. Larsen, C. Erratico, B. Korsgaard and H. Holbech, 2008b, “Vitellogenin as a biomarker for estrogenic effects in brown trout, Salmo trutta: laboratory and field investigations,” Environmental Toxicology and Chemistry, Vol.27, No.11, pp.2387-2396.
    連結:
  12. Blanchet-Letrouvé, I., A. G. Lafont, L. Poirier, S. Baloche and A. Zalouk-Vergnoux, S. Dufour and C. Mouneyrac, 2013, “Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary,” Ecotoxicology and Environmental Safety. Vol.97, pp.103-113.
    連結:
  13. Burki, R., E. L. Vermeirssen, O. Körner, C. Joris, P. Burkhardt‐Holm and H. Segner., 2006, “Assessment of estrogenic exposure in brown trout (Salmo trutta) in a Swiss midland river: integrated analysis of passive samplers, wild and caged fish, and vitellogenin mRNA and protein,” Environmental Toxicology and Chemistry, Vol.25, No.8, pp. 2077-2086.
    連結:
  14. Céspedes, R., S. Lacorte, D. Raldúa, A. Ginebreda, D. Barceló and B. Piña, 2005, “Distribution of endocrine disruptors in the Llobregat River basin,” Chemosphere, Vol.61, No.11, pp.1710-1719.
    連結:
  15. Cadbury, D., 1997, “The feminization of nature: Our future at risk,” Penguin.
    連結:
  16. Caliman, F. A. and M. Gavrilescu., 2009, “Pharmaceuticals, personal care products and endocrine disrupting agents in the environment–a review,” CLEAN–Soil, Air, Water, Vol.37, No.4-5, pp.277-303.
    連結:
  17. Cargouet, M., D. Perdiz, A. Mouatassim-Souali, S. Tamisier-Karolak and Y. Levi., 2004, “Assessment of river contamination by estrogenic compounds in Paris area,” Science of the Total Environment, Vol.324, No.1, pp.55-66.
    連結:
  18. Drastichova, J., Z. Svobodova, M. Groenland, R. Dobšíková, V. Žlábek, D. Weissova and M. Szotkowska., 2005, “Effect of Exposure to Bisphenol A on the Sex Differentiation in Zebrafish (Danio rerio),” Acta Veterinaria Brno, Vol.74, No.2, pp.287-291.
    連結:
  19. Dubé, M. G. and D. L. MacLatchy., 2001, “Identification and treatment of a waste stream at a bleached‐kraft pulp mill that depresses a sex steroid in the mummichog (Fundulus heteroclitus),” Environmental Toxicology and Chemistry, Vol.20, No.5, pp.985-995.
    連結:
  20. Duong, C. N., J. S. Ra, J. Cho, S. D. Kim, H. K. Choi, J.-H. Park, K. W. Kim, E. Inam and S. D. Kim., 2010, “Estrogenic chemicals and estrogenicity in river waters of South Korea and seven Asian countries,” Chemosphere, Vol.78, No.3, pp.286-293.
    連結:
  21. Evans, J. S., L. J. Jackson, H. R. Habibi and M. G. Ikonomou., 2012, “Feminization of Longnose Dace (Rhinichthys cataractae) in the Oldman River, Alberta,(Canada) Provides Evidence of Widespread Endocrine Disruption in an Agricultural Basin,” Scientifica, Vol.2012, pp.1-11
    連結:
  22. ECSCF, 2002, Opinion of the Scientific Committee on Food on Bisphenol A,” EUROPEAN COMMISSION pp.1-22
    連結:
  23. Fenlon, K. A., A. C. Johnson, C. R. Tyler and E. M. Hill., 2010, “Gas-liquid chromatography-tandem mass spectrometry methodology for the quantitation of estrogenic contaminants in bile of fish exposed to wastewater treatment works effluents and from wild populations,” Journal of Chromatography. A, Vol.1217, No.1, pp.112.
    連結:
  24. Fenner, K., C. Kooijman, M. Scheringer and K. Hungerbühler., 2002, “Including transformation products into the risk assessment for chemicals: The case of nonylphenol ethoxylate usage in Switzerland,” Environmental Science and Technology, Vol.36, No.6, pp.1147-1154.
    連結:
  25. García-Reyero, N., D. Raldúa, L. Quirós, G. Llaveria, J. Cerdà, D. Barceló, J. O. Grimalt and B. Piña., 2004, “Use of vitellogenin mRNA as a biomarker for endocrine disruption in feral and cultured fish,” Analytical and Bioanalytical Chemistry, Vol.378, No.3, pp.670-675.
    連結:
  26. Giesy, J. P., E. M. Snyder, K. M. Nichols, S. A. Snyder, S. A. Villalobos, P. D. Jones and S. D. Fitzgerald., 2003, “Examination of reproductive endpoints in goldfish (Carassius auratus) exposed in situ to municipal sewage treatment plant effluent discharges in Michigan, USA,” Environmental Toxicology and Chemistry, Vol.22, No.10, pp.2416-2431.
    連結:
  27. Harries, J. E., D. A. Sheahan, S. Jobling, P. Matthiessen, P. Neall, J. P. Sumpter, T. Tylor and N. Zaman., 1997, “Estrogenic activity in five United Kingdom rivers detected by measurement of vitellogenesis in caged male trout,” Environmental Toxicology and Chemistry, Vol.16, No.3, pp.534-542.
    連結:
  28. Hemmer, M. J., C. J. Bowman, B. L. Hemmer, S. D. Friedman, D. Marcovich, K. J. Kroll and N. D. Denslow., 2002, “Vitellogenin mRNA regulation and plasma clearance in male sheepshead minnows, (Cyprinodon variegatus) after cessation of exposure to 17β-estradiol and nonylphenol ,” Aquatic Toxicology, Vol.58, No.1, pp.99-112.
    連結:
  29. Hintemann, T., C. Schneider, H. F. Schöler and R. J. Schneider., 2006, “Field study using two immunoassays for the determination of estradiol and ethinylestradiol in the aquatic environment,” Water Research, Vol.40, No.12, pp.2287-2294.
    連結:
  30. Hou, L., Y. Xie, G. Ying and Z. Fang., 2011, “Developmental and reproductive characteristics of western mosquitofish (Gambusia affinis) exposed to paper mill effluent in the Dengcun River, Sihui, South China,” Aquatic Toxicology, Vol.103, No.3, pp.140-149.
    連結:
  31. Huang, W., Y. Zhang, X. Jia, X. Ma, S. Li, Y. Liu, P. Zhu, D. Lu, H. Zhao and W. Luo., 2010, “Distinct expression of three estrogen receptors in response to bisphenol A and nonylphenol in male Nile tilapias (Oreochromis niloticus),” Fish Physiology and Biochemistry, Vol.36, No.2, pp.237-249.
    連結:
  32. Isobe, T., H. Shiraishi, M. Yasuda, A. Shinoda, H. Suzuki and M. Morita., 2003, “Determination of estrogens and their conjugates in water using solid-phase extraction followed by liquid chromatography–tandem mass spectrometry,” Journal of Chromatography A, Vol.984, No.2, pp.195-202.
    連結:
  33. USEPA, 1982, “Integrated risk information system of bisphenol A”, CASRN 80-05-7
    連結:
  34. Jeffries, K. M., L. J. Jackson, M. G. Ikonomou and H. R. Habibi., 2010, “Presence of natural and anthropogenic organic contaminants and potential fish health impacts along two river gradients in Alberta, Canada,” Environmental Toxicology and Chemistry, Vol.29, No.10, pp.2379-2387.
    連結:
  35. Jeffries, K. M., L. J. Jackson, L. E. Peters and K. R. Munkittrick., 2008a, “Changes in population, growth, and physiological indices of longnose dace (Rhinichthys cataractae) in the Red Deer River, Alberta, Canada,” Archives of Environmental Contamination and Toxicology, Vol.55, No.4, pp.639-651.
    連結:
  36. Jeffries, K. M., E. R. Nelson, L. J. Jackson and H. R. Habibi., 2008b, “Basin‐wide impacts of compounds with estrogen‐like activity on longnose dace (Rhinichthys cataractae) in two prairie rivers of Alberta, Canada,” Environmental Toxicology and Chemistry, Vol.27, No.10, pp.2042-2052.
    連結:
  37. Jin, S., F. Yang, T. Liao, Y. Hui and Y. Xu., 2008, “Seasonal variations of estrogenic compounds and their estrogenicities in influent and effluent from a municipal sewage treatment plant in China,” Environmental Toxicology and Chemistry, Vol.27, No.1, pp.146-153.
    連結:
  38. Jobling, S., D. Casey, T. Rodgers-Gray, J. Oehlmann, U. Schulte-Oehlmann, S. Pawlowski, T. Baunbeck, A. Turner and C. Tyler., 2004, “Comparative responses of molluscs and fish to environmental estrogens and an estrogenic effluent,” Aquatic Toxicology, Vol.66, No.2, pp.207-222.
    連結:
  39. Jobling, S., J. P. Sumpter, D. Sheahan, J. A. Osborne and P. Matthiessen., 1996, “Inhibition of testicular growth in rainbow trout (Oncorhynchus mykiss) exposed to estrogenic alkylphenolic chemicals,” Environmental Toxicology and Chemistry, Vol.15, No.2, pp.194-202.
    連結:
  40. Kang, J. H. and F. Kondo., 2002, “Bisphenol A degradation by bacteria isolated from river water,” Archives of Environmental Contamination and Toxicology, Vol.43, No.3, pp.265-269.
    連結:
  41. Kim Y. H., Kim C. S., Park S., Han S. Y., Pyo M. Y., and Yang M.,2003, “Gender differences in the levels of bisphenol a metabolites in urine”, Biochemical and Biophysical Research Communications, vol. 312,No.2, pp. 441-448.
    連結:
  42. Kwon, J. Y., F. Prat, C. Randall and C. R. Tyler., 2001, “Molecular characterization of putative yolk processing enzymes and their expression during oogenesis and embryogenesis in rainbow trout (Oncorhynchus mykiss),” Biology of Reproduction, Vol. 65, No.6, pp.1701-1709.
    連結:
  43. Lai, K., K. Johnson, M. Scrimshaw and J. Lester., 2000, “Binding of waterborne steroid estrogens to solid phases in river and estuarine systems,” Environmental Science and Technology, Vol.34, No.18, pp.3890-3894.
    連結:
  44. Lange, A., Y. Katsu, S. Miyagawa, Y. Ogino, H. Urushitani, T. Kobayashi, T. Hirai, J. A. Shears, M. Nagae and J. Yamamoto., 2012, “Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring,” Aquatic Toxicology, Vol.109, pp.250-258.
    連結:
  45. Lange, A., G. C. Paull, P. B. Hamilton, T. Iguchi and C. R. Tyler., 2011, “Implications of persistent exposure to treated wastewater effluent for breeding in wild roach (Rutilus rutilus) populations,” Environmental Science and Technology, Vol.45, No.4, pp.1673-1679.
    連結:
  46. Lech, J. J., S. K. Lewis and L. Ren., 1996, “In vivo estrogenic activity of nonylphenol in rainbow trout,” Toxicological Sciences, Vol.30, No.2, pp.229-232.
    連結:
  47. Lei, B., S. Huang, Y. Zhou, D. Wang and Z. Wang., 2009, “Levels of six estrogens in water and sediment from three rivers in Tianjin area, China,” Chemosphere, Vol.76, No.1, pp.36-42.
    連結:
  48. Li, X., G. G. Ying, J. L. Zhao, Z. F. Chen, H. J. Lai and H. C. Su., 2011, “4-Nonylphenol, bisphenol-A and triclosan levels in human urine of children and students in China, and the effects of drinking these bottled materials on the levels,” Environment International. Vol.52, pp.81-86.
    連結:
  49. Lindholst, C., K. L. Pedersen, S. N. Pedersen., 2000, “Estrogenic response of bisphenol A in rainbow trout (Oncorhynchus mykiss),” Aquatic Toxicology, Vol.48, No.2, pp.87-94.
    連結:
  50. Liu, J., R. Wang, B. Huang, C. Lin, Y. Wang and X. Pan., 2011, “Distribution and bioaccumulation of steroidal and phenolic endocrine disrupting chemicals in wild fish species from Dianchi Lake, China,” Environmental Pollution, Vol.159, No.10, pp.2815-2822.
    連結:
  51. Liu, J., R. Wang, B. Huang, C. Lin, J. Zhou and X. Pan., 2012, “Biological effects and bioaccumulation of steroidal and phenolic endocrine disrupting chemicals in high-back crucian carp exposed to wastewater treatment plant effluents,” Environmental Pollution, Vol.162, pp.325-331.
    連結:
  52. Lopez-Cervantes, J. and P. Paseiro-Losada., 2003, “Determination of bisphenol A in, and its migration from, PVC stretch film used for food packaging,” Food Additives and Contaminants, Vol.20, pp.596-606.
    連結:
  53. Lopez-Espinosa, M., C. Freire, J. Arrebola, N. Navea, J. Taoufiki, M. Fernandez, O. Ballesteros, R. Prada and N. Olea., 2009, “Nonylphenol and octylphenol in adipose tissue of women in Southern Spain,” Chemosphere, Vol.76, No.6, pp.847-852.
    連結:
  54. McClain, J. S., J. T. Oris, G. A. Burton and D. Lattier., 2003, “Laboratory and field validation of multiple molecular biomarkers of contaminant exposure in rainbow trout (Oncorhynchus mykiss),” Environmental Toxicology and Chemistry, Vol.22, No.2, pp.361-370.
    連結:
  55. Metcalfe, C. D., T. L. Metcalfe, Y. Kiparissis, B. G. Koenig, C. Khan, R. J. Hughes, T. R. Croley, R. E. March and T. Potter., 2001, “Estrogenic potency of chemicals detected in sewage treatment plant effluents as determined by in vivo assays with Japanese medaka (Oryzias latipes),” Environmental Toxicology and Chemistry,Vol.20, No.2, pp.297-308.
    連結:
  56. Meucci, V. and A. Arukwe., 2006, “Transcriptional modulation of brain and hepatic estrogen receptor and P450arom isotypes in juvenile Atlantic salmon (Salmo salar) after waterborne exposure to the xenoestrogen, 4-nonylphenol,” Aquatic Toxicology, Vol.77, No.2, pp.167-177.
    連結:
  57. Monteverdi, G. and R. Di Giulio., 1999, “An enzyme-linked immunosorbent assay for estrogenicity using primary hepatocyte cultures from the channel catfish (Ictalurus punctatus),” Archives of Environmental Contamination and Toxicology, Vol.37, No.1, pp.62-69.
    連結:
  58. Nadzialek, S., S. Depiereux, S. Mandiki and P. Kestemont., 2011, “In vivo biomarkers of estrogenicity: limitation of interpretation in wild environment,” Archives of Environmental Contamination and Toxicology, Vol.60, No.3, pp.471-478.
    連結:
  59. Noppe, H., T. Verslycke, E. De Wulf, K. Verheyden, E. Monteyne, P. Van Caeter and C. R. Janssen., 2007, “Occurrence of estrogens in the Scheldt estuary: a 2-year survey,” Ecotoxicology and Environmental Safety, Vol.66, No.1, pp.1-8.
    連結:
  60. Patrolecco, L., S. Capri, S. De Angelis, R. Pagnotta, S. Polesello and S. Valsecchi., 2006, “Partition of nonylphenol and related compounds among different aquatic compartments in Tiber River (Central Italy),” Water, Air, and Soil Pollution, Vol.172, No.1-4, pp.151-166.
    連結:
  61. Paul-Prasanth, B., Y. Shibata, R. Horiguchi and Y. Nagahama., 2011, “Exposure to diethylstilbestrol during embryonic and larval stages of medaka fish (Oryzias latipes) leads to sex reversal in genetic males and reduced gonad weight in genetic females,” Endocrinology, Vol.152, No.2, pp.707-717.
    連結:
  62. Pousis, C., C. De Giorgi, C. Mylonas, C. Bridges, R. Zupa, R. Vassallo-Agius, F. de la Gándara, C. Dileo, G. De Metrio and A. Corriero., 2011, “Comparative study of liver vitellogenin gene expression and oocyte yolk accumulation in wild and captive Atlantic bluefin tuna (Thunnus thynnus),” Animal Reproduction Science, Vol.123, No.1, pp.98-105.
    連結:
  63. Robinson, C. D., E. Brown, J. A. Craft, I. M. Davies and C. F. Moffat., 2004, “Effects of prolonged exposure to 4-tert-octylphenol on toxicity and indices of oestrogenic exposure in the sand goby (Pomatoschistus minutus, Pallas),” Marine Environmental Research, Vol.58, No.1, pp.19-38.
    連結:
  64. Sumpter, J. P. and S. Jobling., 1995, “Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment,” Environmental Health Perspectives, Vol.103, No.Suppl 7, pp.173-178.
    連結:
  65. Tanaka, J. N. and J. M. Grizzle., 2002, “Effects of nonylphenol on the gonadal differentiation of the hermaphroditic fish, Rivulus marmoratus,” Aquatic Toxicology, Vol.57, No.3, pp.117-125.
    連結:
  66. Teo, B. Y., N. S. Tan, E. H. Lim, T. J. Lam and J. L. Ding., 1998, “A novel piscine vitellogenin gene: structural and functional analyses of estrogen-inducible promoter,” Molecular and Cellular Endocrinology, Vol.146, No.1, pp.103-120.
    連結:
  67. Tong, Y., T. Shan, Y. K. Poh, T. Yan, H. Wang, S. H. Lam and Z. Gong., 2004, “Molecular cloning of zebrafish and medaka vitellogenin genes and comparison of their expression in response to 17beta-estradiol,” Gene, Vol.328, pp.25.
    連結:
  68. Vethaak, A. D., J. Lahr, S. M. Schrap, A. C. Belfroid, G. B. Rijs, A. Gerritsen, J. de Boer, A. S. Bulder, G. Grinwis and R. V. Kuiper., 2005, “An integrated assessment of estrogenic contamination and biological effects in the aquatic environment of The Netherlands,” Chemosphere, Vol.59, No.4, pp.511-524.
    連結:
  69. Viganò, L., E. Benfenati, S. Bottero, A. Cevasco, M. Monteverde and A. Mandich., 2010, “Endocrine modulation, inhibition of ovarian development and hepatic alterations in rainbow trout exposed to polluted river water,” Environmental Pollution, Vol.158, No.12, pp.3675-3683.
    連結:
  70. Viganò, L., A. Mandich, E. Benfenati, R. Bertolotti, S. Bottero, E. Porazzi and E. Agradi., 2006, “Investigating the estrogenic risk along the river Po and its intermediate section,” Archives of Environmental Contamination and Toxicology, Vol.51, No.4, pp.641-651.
    連結:
  71. Vom Saal, F. S. and C. Hughes., 2005, “An extensive new literature concerning low-dose effects of bisphenol A shows the need for a new risk assessment,” Environmental Health Perspectives, Vol.113, No.8, pp.926.
    連結:
  72. Voutsa, D., P. Hartmann, C. Schaffner and W. Giger., 2006, “Benzotriazoles, alkylphenols and bisphenol A in municipal wastewaters and in the Glatt River, Switzerland,” Environmental Science and Pollution Research, Vol.13, No.5, pp.333-341.
    連結:
  73. Wang, R., J. Liu, X. Yang, C. Lin, B. Huang, W. Jin and X. Pan., 2013, “Biological response of high-back crucian carp (Carassius auratus) during different life stages to wastewater treatment plant effluent,” Environmental Science and Pollution Research, pp.1-9.
    連結:
  74. Yan, Z., G. Lu, J. Liu and S. Jin., 2012, “An integrated assessment of estrogenic contamination and feminization risk in fish in Taihu Lake, China,” Ecotoxicology and Environmental Safety. Vol.84, pp.334-340.
    連結:
  75. Ying, G. G., R. S. Kookana and Y. J. Ru., 2002, “Occurrence and fate of hormone steroids in the environment,” Environment International, Vol.28, No.6, pp.545-551.
    連結:
  76. Zha, J., L. Sun, Y. Zhou, P. A. Spear, M. Ma and Z. Wang., 2008, “Assessment of 17α-ethinylestradiol effects and underlying mechanisms in a continuous, multigeneration exposure of the Chinese rare minnow (Gobiocypris rarus),” Toxicology and Applied Pharmacology, Vol.226, No.3, pp.298-308.
    連結:
  77. Zhang, H., F. X. Kong, S. H. Wang, Y. Yu and M. Zhang., 2009a, “Vitellogenin induction by a mixture of steroidal estrogens in freshwater fishes and relevant risk assessment,” Environmental Toxicology, Vol.24, No.5, pp.484-491.
    連結:
  78. Zhang, X., Q. Li, G. Li, Z. Wang and C. Yan., 2009b, “Levels of estrogenic compounds in Xiamen Bay sediment, China,” Marine Pollution Bulletin, Vol.58, No.8, pp.1210-1216.
    連結:
  79. Zhang, Z., J. Hu, W. A. Jin, L. An, S. Tao and J. Chen., 2005, “Induction of vitellogenin mRNA in juvenile chinese sturgeon (acipenser sinensis gray) treated with 17β‐estradiol and 4‐nonylphenol,” Environmental Toxicology and Chemistry, Vol.24, No.8, pp.1944-1950.
    連結:
  80. 吳孟純,2012,污水處理廠去除個人防護用品及放流水質對承受水體之評估探討,碩士論文,國立屏東科技大學,環境工程與科學系,屏東。
    連結:
  81. 林子瑜,2005,壬基苯酚誘導人類胎盤滋養層細胞氧化傷害及粒線體功能缺損,碩士論文,台北醫學大學,醫學檢驗暨生物技術學系,台北。
    連結:
  82. 張嘉晃,2006,國人不孕症盛行率調查及環境賀爾蒙壬基苯酚與男性精液品質相關性探討,碩士論文,中國醫藥大學,環境醫學研究所,台中。
    連結:
  83. 郭怡伶,2010,台灣地區主要河川底泥及魚體中壬基酚及雙酚 A 環境流布研究,碩士論文,國立成功大學,環境醫學研究所,台南。
    連結:
  84. 陳至昂,2010,人工溼地內分泌干擾物質之分佈及生態風險之研究,碩士論文,國立屏東科技大學,環境工程與科學系,屏東。
    連結:
  85. 陳玟伶,2012,雌激性化合物在水環境中之流布-方法開發與應用,碩士論文,國立臺灣大學,環境衛生研究所,台北。
    連結:
  86. 溫子瑤,2005,用水處理步驟移除類固醇雌激素之效率,碩士論文,國立台灣大學,環境衛生研究所,台北。
    連結:
  87. 蔣伶霙,2011,台灣地區河川環境水體、底泥、魚體雙酚 A 和壬基酚流布及釋放源解析研究,碩士論文,國立成功大學,環境醫學研究所,台南。
    連結:
  88. Brian, J. V., C. A. Harris, M. Scholze, A. Kortenkamp, P. Booy, M. Lamoree, G. Pojana, N. Jonkers, A. Marcomini and J. P. Sumpter, 2007, “Evidence of estrogenic mixture effects on the reproductive performance of fish,” Environmental Science and Technology, Vol.41, No.1, pp.337-344.
  89. Casey, F. X., G. L. Larsen, H. Hakk and J. Šimunek., 2003, “Fate and transport of 17β-estradiol in soil-water systems,” Environmental Science and Technology, Vol.37, No.11, pp.2400-2409.
  90. Munkittrick, K. R., M. McMaster, G. Van Der Kraak, C. Portt, W. Gibbons, A. Farwell and M. Gray., 2000, “Development of methods for effects-driven cumulative effects assessment using fish populations: Moose River project,” Society of Environmental Toxicology and Chemistry Pensacola, FL, USA.pp.1-256
  91. Nagae, M., Y. Takao, N. Ohkubo, T. Matsubara and K. Soyano., 2010, “Estrogenic Activity in Estuaries by Measuring Serum Vitellogenin Concentration of Japanese Male Common Goby in Northwestern Part of Kyushu,” Coastal Environmental and Ecosystem Issues of The East China Sea, pp.205-213.
  92. Nielsen, E., G. Ostergaard, I. Thorup, O. Ladefoged and J. E. Jelnes., 1999, “Toxicological Evaluation and Limit Values for Nonylphenol, Nonylphenol Ethoxylates, Tricresyl Phosphates and Benzoic Acid,” Environmental Project, No.512, pp.1-43.
  93. Sambrook, J., E. F. Fritsch and T. Maniatis., 1989, “Molecular cloning,” Cold spring harbor laboratory press, New York.
  94. Sanger, F., S. Nicklen and A. R. Coulson., 1977, “DNA sequencing with chain-terminating inhibitors,” Proceedings of the National Academy of Sciences, Vol.74, No.12, pp.5463-5467.
  95. Schmitt, C. J. and G. M. Dethloff., 2000, “Biomonitoring of Environmental Status and Trends (BEST) Program: selected methods for monitoring chemical contaminants and their effects in aquatic ecosystems,” DTIC Document Geological Survey U.S., pp.94
  96. Servos, M. R., 1999, “Review of the aquatic toxicity, estrogenic responses and bioaccumulation of alkylphenols and alkylphenol polyethoxylates,” Water Quality Res J Canada, Vol.34, No.1, pp.123-177.
  97. Snyder, S., 2003, “Endocrine disruptors as water contaminants: Toxicological implications for humans and wildlife,” Southwest Hydrology, Vol.2, No.6, pp.14.
  98. Specker, J. and C. Sullivan., 1994, “Vitellogenesis in fishes: status and perspectives,” Perspectives In Comparative Endocrinology, pp. 304-315.
  99. Spivack, J., T. Leib and J. Lobos., 1994, “Novel pathway for bacterial metabolism of bisphenol A. Rearrangements and stilbene cleavage in bisphenol A metabolism,” Journal of Biological Chemistry, Vol.269, No.10, pp.7323-7329.
  100. Tabata, A., S. Kashiwada, Y. Ohnishi, H. Ishikawa, N. Miyamoto, M. Itoh and Y. Magara., 2001, “Estrogenic influences of estradiol-17 beta, p-nonylphenol and bis-phenol-A on Japanese medaka (Oryzias latipes) at detected environmental concentrations,” Water Science and Technology: a Journal of the International Association on Water Pollution Research, Vol.43, No.2, pp.109.
  101. 丁望賢,吳建誼,2000,「環境荷爾蒙-壬基苯酚與雙酚 A 在台灣水環境中之分析與流布調查」,環境檢驗雙月刊,第33期,第12-21頁。
  102. 刈米孝夫,1996,界面活性劑的原理與應用,高立圖書有限公司,王鳳英譯,台北。
  103. 王正雄,王小萍,李宜樺,黃壬瑰,陳佩珊,洪文宗,2000,「台灣地區擬似環境荷爾蒙物質管理及環境流布調查」,微生物與環境荷爾蒙研討會,第1-25頁。
  104. 王朝暉,王奕捐,2008,「環境激素類物質對人類健康的影響」,生態科學,第26卷,第6期,第564-569頁。
  105. 王漢泉,2002,「台灣河川水質魚類指標之研究」,環境檢驗所環境調查研究年報,第9卷,第207-236頁。
  106. 江政哲,2002,以固相萃取法及氣相層析質譜儀對水環境中動情激素類有機污染物之分析與研究,碩士論文,國立中央大學,化學研究所,桃園。
  107. 行政院衛生署,2007,「食品用洗潔劑衛生標準」,衛署食字第0960404499號令。
  108. 行政院衛生署,2010,「食品器具容器包裝衛生標準」,衛署食字第0991303265號令。
  109. 行政院環保署,2010,「環境荷爾蒙管理計畫」,台灣。
  110. 李美慧,2003,「利用雄孔雀魚評估環境雌性激素之影響」,行政院國家科學委員會專題研究計畫成果報告,計畫編號: NSC91-2313-B-002-395。
  111. 李美慧,2012,「環境荷爾蒙與嬰幼兒的健康」,科學發展,第470期,第58-63頁。
  112. 李婉萍,2003,人體血漿中壬基苯酚、辛基苯酚及丁基苯酚之同時測定方法研究,碩士論文,國立陽明大學,環境衛生研究所,台北。
  113. 林祁麒,2003,可怕的環境荷爾蒙,安立出版社,第88-105頁。
  114. 林家進,2010,應用琵琶鼠魚(Pterygoplichthys multiradiatus)監測水中類雌性素-以可氯丹、靈丹、雙酚A、壬基苯酚為例,碩士論文,國立高雄海洋科技大學,海洋環境工程研究所,高雄。
  115. 范綱閔,2008,藥物及個人防護產品於武洛溪人工溼地之分佈與去除率探討,碩士論文,國立屏東科技大學,環境工程與科學系,屏東。
  116. 洪鳴遠,2005,壬基苯酚對雄性劍尾魚(Xiphophorus hellerii)生精作用之影響,碩士論文,國立屏東科技大學,獸醫學系,屏東。
  117. 消費者文教基金會,2012,「掃「塑」-18%的感熱紙檢出雙酚A」,消費者報導-消費柯南,第374期,第38-44頁。
  118. 張志豪,2008,環境荷爾蒙-己烯雌酚對念珠菌影響之研究,碩士論文,國立屏東教育大學,應用化學暨生命科學系,屏東。
  119. 曹勁松,李意,2005,「初乳、常乳及其製品中的雌性激素」,中國乳品工業,第9期,第33卷,第4-8頁。
  120. 陳珮珊,2000,烷基酚類化合物對雄性鯉魚卵黃前質生成之影響,碩士論文,國立陽明大學,環境衛生研究所,台北。
  121. 陳健民,2003,「以吳郭魚探討二仁溪水仿雌激素活性的探討」,嘉南藥理科技大學專題研究計畫成果報告,計畫編號:CNEV92-05。
  122. 陳榮圻,2006,「烷基酚聚氧乙烯醚 (APEO) 的禁用和代用」,印染,第32期,第45-49頁。
  123. 陳錫金,2005,界面活性劑 Octylphenol Polyethoxylates 生物降解與復育之研究,博士論文,國立中央大學,環境工程研究所,桃園。
  124. 國立屏東科技大學,2012,「高屏溪第二次河川情勢調查」,經濟部水利署第七河川局,台灣
  125. 黄曄,任華,孫竹筠,周忠良,2008,「壬基酚和雙酚 A 對雄性斑馬魚(Danio rerio)卵黄蛋白原mRNA的誘導效應」,生態毒理學報,第3卷,第3期,第 274-279頁。
  126. 黃妙如,2010,雙酚 A 對斑馬魚各生長階段之毒性影響,碩士論文,國立雲林科技大學,防災與環境工程所,雲林。
  127. 楊友仁,2004,都市水體中類雌激素化合物之分析,碩士論文,大仁科技大學,環境管理研究所,屏東。
  128. 潘弋戎,2004,利用吳郭魚卵黃蛋白前質誘導量檢測都市污水處理廠放流水的雌激素活性,碩士論文,嘉南藥理科技大學,環境工程與科學系,台南。
Times Cited
  1. 陳愉錡(2014)。南部溪流水體、底泥、魚體中雌激素類化合物的分佈及生物累積之探討。屏東科技大學環境工程與科學系所學位論文。2014。1-84。