Title

補骨脂活性成份 Neobavaisoflavone 促進造骨細胞分化及礦化之機轉研究

Translated Titles

Effect of Neobavaisoflavone on the Cell Differentiation and Mineralization of MC3T3-E1 Preosteoblasts:Evaluating its Mechanisms of action

DOI

10.6836/NTTU.2011.00343

Authors

吳欣諭

Key Words

造骨細胞 ; 新補骨脂異黃酮 ; 鹼性磷酸酶活性 ; 礦化 ; osteoblast ; neobavaisoflavone ; alkaline phosphatase activity ; mineralization

PublicationName

臺東大學生命科學系碩士班學位論文

Volume or Term/Year and Month of Publication

2011年

Academic Degree Category

碩士

Advisor

邱文慧,魏百祿

Content Language

繁體中文

Chinese Abstract

補骨脂 (Psoralea corylifolia L.)為一已知傳統中藥,已被用來補骨、壯陽或是治療骨折。研究顯示補骨脂粗萃取物不僅會刺激造骨細胞分化,也可改善去卵巢鼠的骨質疏鬆。Neobavaisoflavone(NBIF)、Psoralidin、Psoralen、及Isopsoralen 是由補骨脂所分離出來的 4 個純化物,但其在造骨細胞的活性作用尚未釐清。因此,本論文利用前驅造骨細胞株 MC3T3-E1 做更進一步探討,進行篩選試驗中,我們發現 NBIF 具有最強的活性,可以刺激細胞分化時 ALP 活性的增加。本研究結果顯示 NBIF 呈濃度相關的增加 ALP 活性及礦化作用。而給予 p38 MAPK 抑制劑 SB203580 和 phosphatidylinositol 3-kinase (PI3K)/Akt 抑制劑 wortmannin 亦顯著抑制 NBIF 所誘導之 ALP 活性,而 extracellular signal-regulated protein kinases(ERK)抑制劑 PD98059、c-Jun NH2 terminal kinases(JNK)抑制劑 SP600125 及 estrogen receptors 抑制劑 ICI 182,780 則不影響 ALP 活性。NBIF 亦可以增加 p38 MAPK 和 PI3K/Akt 磷酸化。另外,NBIF 甚至增加造骨轉錄因子 runt-related transcription factor 2、osterix,骨基質 osteonectin、bone sialoprotein 和骨形成蛋白 bone morphogenetic protein-2 mRNA 表現的產生,而這些現象皆會被 p38 MAPK 抑制劑和 PI3K/Akt 抑制劑所阻斷。因此 NBIF 增加造骨細胞分化的機轉可能與 p38 MAPK 和 PI3K/Akt 訊號路徑相關。

Topic Category 理工學院 > 生命科學系碩士班
生物農學 > 生物科學
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