利用被動式生物通氣系統整治柴油風化污染土壤

Using Passive Bioventing System to Remediate Diesel-Contaminated Weathering Soil

王嘉成(JIA-CHENG WANG)

指導教授 ： 劉敏信

朝陽科技大學/理工學院/環境工程與管理系/碩士(2017年)

摘要

生物復育法是利用自然微生物降解污染物，具低操作成本、無二次污染等優點，而生物通氣法是生物復育法其中一種方法。本研究使用某場址風化後柴油污染土壤，殘餘濃度約5,000 mg/kg設置模場，套用自製被動式風力驅動通氣設備，利用自然風驅動系統，使得自然風進入土壤層，讓土壤層中原有微生物或引進的微生物在高氧氣濃度的環境下，進行好氧分解有機污染物。本試驗使用風化後柴油污染土壤，試驗過程中個別添加具柴油降解能力之三種微生物及適量營養鹽，添加前先以少量柴油馴化微生物，試驗組分別添加Bacillus cereus菌、Achromobacter xylosoxidans菌、Pseudomonas putida菌，研究結果顯示於試驗210日後各通氣組皆呈現明顯柴油降解趨勢，各添加單一菌株組之柴油降解率皆達到85%以上，而添加Bacillus cereus菌組採用Mann Kendall Test統計判斷Z值為-3.00，再此判斷柴油污染物降解效果最為明顯。
試驗中同時以氣泡式呼吸儀於降解期間對各組別進行土壤微生物呼吸試驗，將每週批次試驗5日累積攝氧率統計分析，做為判斷微生物活性的指標及添加營養鹽的時機，有助於受柴油污染土壤之整治。

關鍵字

被動式生物通氣法 ； 氣泡式呼吸儀 ； 柴油降解菌 ； 生物整治

並列摘要

Bioremediation utilizes natural microorganisms to degrade contaminants, with low operating costs and limit secondary pollution reveal its advantages, and bioventing is one of such bioremediation processes. In this study, the weathered diesel-contaminated soil of a polluted site with a residual concentration of around 5,000 mg/kg was placed in a simulated polluted field with self-made passive wind-driven bioventing equipment. Using a natural wind-driven system, wind was induced into the soil layer, causing native or introduced microorganisms inside to aerobically degrade organic contaminants under high oxygen concentrations. Experiments were conducted by adding three microorganisms with diesel degrading abilities each into the weathered diesel-contaminated soil sets along with suitable amounts of nutrients. The microbes, namely Bacillus cereus, Achromobacter xylosoxidans and Pseudomonas putida were first domesticated with small amounts of diesel prior to addition. Results after 210 days of experimentation showed that all bioventing sets have distinct trends of diesel degradation. Each set added with a single strain of microbes had a degradation rate of 85% and above. The Z-value obtained from the Mann Kendall Test of the Bacillus cereus set was -3.00, further determining that it has the most distinct degrading effect on diesel contaminants.
Simultaneously, a bubble respirometer was used to conduct microbial respiration tests for each set during the degradation phase. Statistical analysis was conducted by using 5-day of accumulated oxygen uptake from weekly batch experiments. Results can be used as microbial activity indicators as well as to determine the timings for nutrient addition, which is useful for the remediation of diesel-contaminated soil.

並列關鍵字

Passive bioventing ； bubble respirometer ； diesel degrading microorganisms ； bioremediation

參考文獻

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