以MALDI-TOF質譜分析牛樟芝中之生物性成分

Analysis of Bioactive Components by MALDI-TOF MS in Antrodia cinnamomea

郭朝正

金奈米粒子 ； MALDI-TOF MS ； 醌類衍生物 ； 三萜類 ； 牛樟芝 ； Ubiquinone derivatives ； Triterpenoids ； Antrodia cinnamomea ； MALDI-TOF MS ； Gold nanoparticles

朝陽科技大學應用化學系生化科技碩博士班學位論文

2017年

碩士

曾耀銘;劉炳嵐

繁體中文

研究指出，牛樟芝(Antrodia cinnamomea)內最受注目之三萜類化合物具有抑制腫瘤細胞、提升免疫系統、肝臟保護作用以及抗氧化能力等活性效用；近年來，醌類衍生物也漸漸成為最具生物活性的成分之一，也具有治療癌症、男性不育和心血管疾病等活性。本實驗利用MALDI-TOF MS建立三萜類化合物與醌類衍生物之快篩方法。研究中主要利用奈米材料檸檬酸鈉修飾金奈米粒子(Citrate-capped gold nanoparticles, AuNPs)作為基質，其具有聚集沉澱之特性，亦可達到濃縮的效果並提高訊號強度與再現性，分析低分子量物質可有效降低背景基質訊號干擾。目前已鑑定出9種三萜類化合物：Methyl antcinate B (AC1)、Antcin B (AC5)、Dehydrosulphurenic acid (AC6)、Antcin H (AC7)、Antcin A (AC9)、Antcin C (AC10)、Methyl antcinate A (AC11)、Antcin K (AC12)、Methyl antcinate H (AC13)以及醌類衍生物4-acetylantroquinonol B (4AAQB)，建立出AC1、AC5、AC6、AC7、AC9、AC10、AC11、AC12、AC13、4AAQB之檢量線，其線性範圍 1~20 ppm (r2 >0.98)。此種方法可快速區分與大量分析不同牛樟芝系列樣品，進而比較子實體和菌絲體內生物性成分之差異。

Antrodia cinnamomea (AC) is a unique mushroom of Taiwam. Triterpenoids and ubiquinone derivatives possess a variety of biological activities such as anticancer, antimicrobial, anti-inflammatory, antioxidant, and hepatoprotive. In the present study, citrate-capped gold nanoparticles (AuNPs) was utilized as a matrix for the analysis of triterpenoids and ubiquinone derivatives by MALDI-TOF/MS. AuNPs are deposited onto the top of the sample layer, resulting in a increase of the signal intensities of the protonated ions and reproducibility. The method has been successfully employed to measured 9 triterpenoids and ubiquinone derivatives in A. cinnamomea. The identified analytes include methyl antcinate B (AC1), antcin B (AC5), Dehydrosulphurenic acid (AC6), antcin H (AC7), antcin A (AC9), antcin C (AC10), methyl antcinate A (AC11), antcin K (AC12), methyl antcinate H (AC13), and 4-acetylantroquinonol B (4AAQB). The results, showing the linear range and r2 value are 1 ppm~20 ppm with r2 = 0.957 ~ 0.985, respective. This method can fast distinguish the different sources of A. Cinnamomea. Comparison bioactive components between fruiting body and mycelium.

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