新型牙周再生膜之研製與評估

Preparation and Evaluation of A Novel Periodontal Regeneration Membrane

張惟閎(Wei-hong Chang)

指導教授 ： 吳世經

中臺科技大學/健康科學院/醫學工程暨材料研究所/碩士(2008年)

摘要

本研究目的為?用靜電紡絲技術應用於新型牙周再生膜之研製與評
估，期望在靜電紡絲技術的應用下，所製備出的?米纖維結構能夠具有促
進骨細胞貼附與增生的效果，藉此進一步達到牙周組織的修?。本研究?
用靜電紡絲技術製備出?米表面結構之牙周再生膜，而靜電紡絲於材?上
的選擇主要為明膠（gelatin），再與聚乙烯醇Poly（Vinyl Alcohol）比?為
9:1 之?混後溶液做為靜電紡絲所需的高分子溶液。高分子溶液經由靜電
紡絲製出的結構直徑大小藉由掃描式電子顯微鏡（Scaning Electronic
Microstructure, SEM）觀察約為145±6.1 nm；再經由15、45、90 和360 分
鐘?同時間的戊二醛（glutaraldehyde；GA）蒸氣交??固定紡絲的?米
網?結構。而經過GA蒸氣交?後的直徑大小約為279.2±20 nm至403.2±21
nm。交?後，經過浸泡去?子水七天後之直徑變化為575.6±6 至430±15 nm
即可證實製備的?米纖維確實有達到交?的效果。材?特性分析方面，以
熱差分析與傅??紅外線光譜儀?進?分析，而經由傅??紅外光譜儀分
析的結果顯示，可以於峰值1622cm-1處顯示出交?後所產生的新的官能
基；再經過熱差分析儀進一步證實?同交?時間對於材?的交?，玻璃轉
移溫?確實因材?經過交?後有增加的趨勢。在生物適應性方面，所得的
結果顯示，經由靜電紡絲所製備出?的纖維於短時間的細胞培養下，材?
表面結構經由GA交?後，由細胞存活?與細胞貼附測試結果得知45 分鐘
為最佳交?時間；最後進一步由免疫螢光染色證實具?米結構材?表面確
米結構材?表面確實可以達到促進骨細胞貼附的效果。

關鍵字

靜電紡絲 ； 引導組織再生 ； 明膠

並列摘要

The objective of this study was to prepare Gelatin/PVA nanofibers
via electrospinning as a novel GTR membrane. After sample preparing,
the surface morphology of electrospun membranes was observed by using
scanning electron microscopy (SEM). Afterwards, electrospun
membranes were crosslinked by using glutaraldehyde (GA) vapor at
different crosslinking conditions. The crosslinking property of membrane
was characterized by differential scanning calorimetry (DSC), membrane
swelling and fourier transform infrared spectrometry (FTIR). The effect
of crosslinked membrane on cell growth was investigated by study of
osteoblastic viability and attachment.
The result showed that the average diameter of nanofibers in
membrane was around 145±6.1 nm. After crosslink treatment, the
diameter was increasing to around 279.2±20 nm to 403.2±21 nm. In
addition, results of DSC and FTIR showed that new chemical linkage
formed and physical property of membrane changed. A new functional
group was detected at 1622 cm-1. The glasstransition temperature (Tg) of
membrane was increasing from 99.26 to 110.76 oC. Different crosslinked
membranes were soaked in deionization water for 7 days to study the
effect of crosslinking on swelling of membrane. The diameter was
increasing from 575.6±6 nm to 430±15 nm. The result of cell viability
and attachment showed that the structure of nanofibers can promote cell
attachment rate. Confocal study also proved the same result.

並列關鍵字

electrospinning ； gelatin ； guide tissue regeneration

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