三價鉻離子增強Luminol-KIO4之化學發光性質研究與應用

黃秋鈴

doi:10.6342/NTU.2011.00249

184.72.102.217，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

Basic & Applied Sciences > Chemistry
College of Science > Graduate Institute of Chemistry

Bibliography Management Tool

Related Links

Report an Issue

Purchase Single Articles

Download PDF

三價鉻離子增強Luminol-KIO4之化學發光性質研究與應用

Enhancement of Chemiluminescence of the Luminol-KIO4 System by Cr(III) and Its Applications

黃秋鈴 , Masters　　Advisor：林萬寅　　

繁體中文 DOI： 10.6342/NTU.2011.00249

流動注入分析法；三價鉻離子；活性含氧物質；苯二酚；兒茶酚胺；苯三酚；色胺酸； flow injection analysis ； chromium(III) ion ； reactive oxygen species ； benzenediol ； catecholamines ； benzenetriol ； tryptophan

Abstract │ Reference (49) │ Altmetrics

Parallel Abstract 〈TOP〉

We have studied the enhancement in CL for luminol-KIO4 system caused by chromium(III) ion using flow injection analysis. For the KIO4/ luminol CL systm﹐about 100-fold increase in CL intensity was observed upon addition of 150 μM Cr(III) at pH 13. The effect of concentration of reagents (chromium(III)﹐luminol﹐potassium periodate)﹐pH﹐flow rate﹐and modes of reagent mixing on CL emission were also investigated and optimized. 
In emission spectrum, the CL maximum occurred at 429 nm. So the CL is caused by luminol in Cr(III)/ KIO4/ luminol system. When the dissolved oxygen was removed from the solution by purging with nitrogen﹐the CL decreased by 20 %. This might indicate that superoxide ion played a minor role in this CL reaction. In order to know the mechanism of the CL of Cr(III)/ KIO4/ luminol system, we tested a series of free radical scanvengers for their effect on CL emission. The CL intensity decreased greatly in the presence of these scavengers. These results showed that in addition to •O2－, 1O2 and •OH also participated in CL reaction. The CL-enhancement may result from the reaction of KIO4 and dissolved oxygen in strong alkaline solution to produce reactive oxygen species (ROS) in the prescence of catalytic Cr3+. However, the major reason for CL-enhancement would be the formation of LuH-Cr-IO4 complex that increases the oxidation of luminol. 
By using the CL system, we tested 44 compounds for their effect on the CL emission, including amino acids, polyphenol, catecholamine, nitrogenous bases, vitamins, etc. The results showed that the system has high selectivity for tryptophan. 
Furthermore, we found that common antioxidants such as benzenediols and catecholamines showed inhibiting effect on our CL system because these compounds might eliminate •O2－. On the contrary, the CL emission increased at low concentration of benzenetriol because of the generation of more •O2－ upon oxidation of benzenetriol .Nevertheless, the CL emission decreased at high concentration. 
Finally we did a series of optimization for the best inhibitory effect of hydroquinone and catechol, to achieve low detection limits of 1.02 nM and 0.96 nM respectively.

Reference ( 49 ) 〈TOP〉

2. Qin, W. Anal. Lett. 2002, 35, 2207-2220.
連結：
3. Campbell, A. K. In Chemiluminescence: Principles and Applications in Biology and Medicine. New York, 1988, pp 19-67.
連結：
5. Seitz, W. R.; Neary, M. P. Anal. Chem. 1974, 46, 188-200.
連結：
6. Wilson, E. Chem. Eng. News 1999, 77, 65-65.
連結：
10. Safavi, A.; Karimi, M. A. Anal. Chim. Acta 2002, 468, 53-63.
連結：

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：