利用細胞模式評估Bracteanolide A的抗氧化生物功能

游雅茹

doi:10.6342/NTU.2012.00333

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利用細胞模式評估Bracteanolide A的抗氧化生物功能

Investigate the anti-oxidant effects of Bracteanolide A by cell model

游雅茹 , Masters　　Advisor：謝淑貞　　

繁體中文 DOI： 10.6342/NTU.2012.00333

Abstract │ Reference (165) │ Times Cited (2) │ Altmetrics

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Oxidative stress will contribute to the accumulation of ROS, and result in many chronic diseases, such as cancers, cardiac diseases, neurodegenerative diseases, and aging etc. via damaging DNA, proteins, and cellular membranes in the body. Therefore, intaking the dietary supplement containing antioxidants is gradually becoming a portion of modern life. However, the researches about the antioxidant functions always merely focus on the in vitro chemical reactions, for example, DPPH radical scavenging activity, chemical colorimetric methods using Ammonium thiocyanate and iron (II) chloride tetrahydrate solution, and so forth. If we can analyze these materials by the ways which are more closed to the actual organism, the antioxidant functions of them can be more convinced.    
Nuclear factor-E2 (Erythroid 2)-related factor 2, Nrf-2, is a transcription factor and also an important regulation factor of antioxidation pathway. When our bodies are damaged by oxidative stress, the factor can translocate into the nucleus and binds to the antioxidant response element (ARE) to induce the expression of downstream genes, including many related enzymes and proteins about antioxidation, and then act to reduce oxidative damages. At first, the study utilizes RT-q-PCR as a screening method to detect the expression of the downstream gene- NQO1. Finally, we discovered that Bracteanolide A , the pure compound contained higher in Tradescantia albiflora Kunth, at the concentration of 50 μM can apparently induce the expression of NQO1 mRNA among the 11 compounds. Other experiments also indicate the compound has the ability to induce the expression of Nrf2, NQO1, and HO-1 on the protein level. Besides, it can increase the amount of Nrf2 which translocates into the nucleus to elevate its activity. If pretreating the cells with Bracteanolide A, ROS induced by hydrogen peroxide can be effectively reduced and DNA damages will be strongly lowered. Last but the most important, it can inhibit the cell death caused by hydrogen peroxide. Overall, the main component of Tradescantia albiflora Kunth, Bracteanolide A, possesses the protective effect on oxidative damaged cells.

Reference ( 165 ) 〈TOP〉

莊雅玲 (2005)，白花水竹草降低大鼠血中尿酸值、抗氧化性及其毒性探討。國立台灣大學獸醫學研究所碩士論文。
連結：
曾瀚群 (2008)，白花水竹草之化學成份研究。國立台灣大學理學院化學系碩士論文。
連結：
Abraham, N. G., Lavrovsky, Y., Schwartzman, M. L., Stoltz, R. A., Levere, R. D., Gerritsen, M. E., Shibahara, S., & Kappas, A. (1995). Transfection of the human heme oxygenase gene into rabbit coronary microvessel endothelial cells: protective effect against heme and hemoglobin toxicity. Proc Natl Acad Sci U S A, 92(15), 6798-6802.
連結：
Andersen, J. K. (2004). Oxidative stress in neurodegeneration: cause or consequence? Nature Medicine, 10(7), S18-S25.
連結：
Andrews, B. T., Paganga, G. T., & Rice-Evans, C. A. (1993). Erythrocyte-induced oxidation of low density lipoproteins: control by antioxidant nutrients. Biochem Soc Trans, 21(2), 94S.
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