透過您的圖書館登入
IP:3.83.81.42
  • 學位論文

靈芝子實體之奈米/次微米粒子的製備與特性鑑定

Preparation and Characterization of Nano/Submicron Particles from Ganoderma Fruiting Bodies

指導教授 : 葉安義

摘要


本研究利用介質研磨技術,製備奈米/次微米尺度之松杉靈芝 (Ganoderma tsugue) 子實體顆粒懸浮液,並探討其穩定性及活性物質含量。松杉靈芝子實體經高速均質處理,再以0.8、0.3及0.2 mm之研磨介質分別研磨60分鐘,最後利用離心(10,000 g, 10 min)去除靈芝子實體研磨懸浮液中的大顆粒,可得具奈米/次微米子實體微粒之離心上清液 (supernatant III)。以雷射粒徑分析儀分析粒徑分佈,並以穿透式電子顯微鏡驗證粒徑檢測結果。離心上清液中單一粒子之粒徑均可達100 nm以下,但因粒子之再聚集使其成為粒徑為數百奈米之微粒圑簇(cluster)。移除懸浮液中之大顆粒,可提高其穩定性﹔於25oC下貯存21天後,離心上清液之體積平均粒徑只由0.105 至0.137 μm。離心上清液經冷凍乾燥後,顆粒會聚集而形成大顆粒。將離心上清液之凍乾試樣再分散於水溶液中並測定粒徑分佈,其體積平均粒徑大於1 μm,並只有近 20% 的顆粒小於100 nm﹔高溫高壓滅菌處理對於離心上清液之穩定性並無嚴重影響,於25oC下貯存12個月後,仍有約 40% 的顆粒小於100 nm,體積平均粒徑則只增至0.373 μm。介質研磨的產物中之β-(1,3)-D-glucans、粗三萜類、總膳食纖維及幾丁質等活性成分含量均高於熱水萃出物。靈子奈米/次微米粒子懸浮液之抗氧化能力雖不及熱水萃出物,但在較高濃度下確實具有抗氧化功效,由抗氧化成分分析結果可知,抗氧化功效應主要來自研磨過程中釋出的酚類化合物。以Supernatnat III處理72小時,可有效抑制人類肝癌細胞株HepG2及皮膚癌細胞株A375之細胞存活率。

並列摘要


The preparation and stability of nano/submicrometer-sized Ganoderma tsugae (G. tsugae) was explored. The fruit body of G. tsugae was treated by homogenization and media-milling. Three sizes (0.8, 0.3, and 0.2 mm) of media were employed for three consecutive milling (I, II and III). The supernatant was considered as final product after centrifuge (10,000 g, 10 min). The removal of large particles resulted in better stability. Particle size was determined by using a laser-light particle size analyzer and further examined by using transmission electron microscopy. After being stored at 25oC for 21 days, the volume-average diameter of supernatant III remained below 0.2 μm with good stability. Freeze-drying resulted in an increase in particle size due to aggregation. Twenty percents of the particles in the freeze-dried supernatant III were smaller than 100 nm, with an average diameter greater than 1 μm. Nevertheless, autoclaving did not result in severe instability of supernatant III. After being stored at 25oC for 12 months, about 40% of the particles were smaller than 100 nm, with an average diameter of 373 nm. In addition, the contents of bioactive compounds such as β-(1,3)-D-glucans, crude triterpenoids, total dietary fiber and chitin in media-milled products were much greater than those present in hot-water extract. The media-milled products from G. tsugae showed moderate antioxidant activities at high concentration, and total phenols were the major naturally occurring antioxidant component. The anti-proliferation effect was observed in vitro growth of HepG2 and A375 cell lines after incubation with Supernatant III.

參考文獻


劉盈吟,2007,奈米/次微米化纖維素之特性及安全性,國立台灣大學食科技研究所碩士論文。
陳時欣,2006,蔗糖酯對奈米/次微米纖維素懸浮液穩定性之研究,國立台灣大學食科技研究所碩士論文。
許瑞婷,2005,研磨對臭氧降解纖維素的影響,國立台灣大學食科技研究所碩士論文。
趙明煜,2004,奈米纖維製備方法之研究,國立台灣大學食科技研究所碩士論文。
Zhang NL, Zhang SY. 1997. Analysis of vitamins and polysaccharides of Gaoderma spores. Chin J Biochem Pharmacol 18: 37 – 39.

被引用紀錄


黃琳元(2016)。奈米�次微米靈芝懸浮液多醣區分物對巨噬細胞活化之影響〔碩士論文,國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342%2fNTU201603579
江怡嫻(2015)。奈米/次微米靈芝懸浮液與其功能性成分對免疫調節之影響〔博士論文,國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342%2fNTU.2015.11044

延伸閱讀