以法呢焦磷酸酯螢光類似物探測十一異戊二烯焦磷酸合成酶的活性中心和反應構形變化

李育瑾

3.239.242.55，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

Basic & Applied Sciences > Chemistry
College of Science > Graduate Institute of Chemistry

Bibliography Management Tool

Related Links

Report an Issue

Search in Library

There is no full-text available at the moment. Airiti would like to request author's authorization. I would like to provide authorization.

以法呢焦磷酸酯螢光類似物探測十一異戊二烯焦磷酸合成酶的活性中心和反應構形變化

Probing the Active Site and Conformational Change of Undecaprenyl Pyrophosphate Synthase by Farnesyl Pyrophosphate Fluorescent Analogues

李育瑾 , Masters　　Advisor：陳昭岑　　

繁體中文

法呢焦磷酸酯螢光類似物；十一異戊二烯焦磷酸合成酶； Farnesyl Pyrophosphate Fluorescent Analogues ； Undecaprenyl Pyrophosphate Synthase ；十一異戊二烯焦磷酸合成酶； Farnesyl Pyrophosphate Fluorescent Analogues ； Undecaprenyl Pyrophosphate Synthase

Abstract │ Reference (57) │ Times Cited (4) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Undecaprenyl pyrophosphate synthase (UPPs) of E. Coli. catalyzes the condensation of eight molecules of isopentyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to generate C55 undecaprenyl pyrophosphate, which serves as a carrier in transporting lipid II across the membrane in bacterial cell wall biosythesis.
    We successively synthesized four FPP fluorescent analogues: 14 (NBD-FPP), 24 (fluorescein-FPP), 34 (MANT-ESTER-FPP) and 44 (MANT-AMIDE-FPP). We obtained spectroscopic characterization of four analogues by steady-state fluorescence spectrophotometer, and we utilized those to observe the ligand interactions with UPPs. And we also got different kinetic parameters of four analogues. 24 has the best competitive inhibition, and when binding with UPPs, the difference of fluorescent intensity can be seen by eyes. 34 can serve as alternative substrate for UPPs, and could be used as drug screening. Analogues 14, 24, 34, 44 could be used to study different FPP-binding proteins and served as high throughput probes.

Reference ( 57 ) 〈TOP〉

1. Poulter, C. D.; Rilling, H. C. Biosynthesis of Isoprenoid Compounds (Spurgeon, S.L., ed.) John Wiley & Sons, New York. 1981.
連結：
2. Poulter, C. D. J. Agric. Food Chem. 1974, 22, 167-173.
連結：
3. Sinensky, M. Biochim. Biophys. Acta. 2002, 1484, 93-106.
連結：
7. Robyt, J. Essentials of Carbohydrate Chemistry. Springer-Verlag, New York. 1998.
連結：
8. Casey, P. J. Biochem. Soc. Trans. 1995, 23, 161-166.
連結：

Times Cited (4) 〈TOP〉

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：