超高效能液相層析電灑質譜法分析乙型受體素之研究與探討

張璇璽

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超高效能液相層析電灑質譜法分析乙型受體素之研究與探討

Determination of β-agonists Using Ultra High Performance Liquid Chromatography Mass Spectrometry

張璇璽 , Masters　　Advisor：何國榮　　

繁體中文

超高效液相層析法；電灑法質譜；乙型受體素類；管柱後分流； UPLC ； ESI/MS ； β-agonists ； post-column splitting

Abstract │ Reference (57) │ Times Cited (1) │ Altmetrics

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The ultra performance liquid chromatography (UPLC) is based on the principle of using stationary phase with particles less than 2 μm. According to the Van Deemter equation, as the particle size decreases to less than 2 μm, not only increased efficiency, but also the ability to work at higher linear velocity without a loss of efficiency, providing both resolution and speed. The flow rate of mobile phase is an important parameter affecting the sensitivity of LC-ESI-MS. High flow rate can reduce the ionization efficiency. Therefore, low flow rate was often used. However, low flow rate failed to achieve column efficiency and rapid analysis.

In this study, post-column splitting was used to reduce the flow into the mass spectrometer. This would enhance the ionization efficiency under ESI. First part of this work was to compare the sensitivity at different flow rates. The results showed that flow rate at 0.2 mL/min provided the best sensitivity. Therefore, the flow rate of mobile phase was at to 0.6 mL/min. Using post-column splitting (split ratio1:3), a flow rate of 0.2 mL/min was introduced into the ESI-MS system for detection.

UPLC-ESI-MS/MS with post-column splitting (0.2 mL/min) provided better sensitivity than elution flow rate of 0.6 mL/min without splitting. However, post-column splitting did not achieve the sensitivity under 0.2 mL/min without splitting. One possible reason is the limitation in the rate of transferring the ions from ESI source to the mass spectrometer.

Reference ( 57 ) 〈TOP〉

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9. Liu, W.; Zhang, L.; Wei, Z. Y.; Chen, S. P.; Chen, G. N. J. Chromatogr. A. 2009, 1216, 5340.
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Times Cited (1) 〈TOP〉

游孟儒(2015)。動物用藥於液相層析電灑質譜之訊號改善研究。臺灣大學化學研究所學位論文。2015。1-116。

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