以單分子方法探討人類Dmc1蛋白在進行DNA股交換時的核蛋白結構

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以單分子方法探討人類Dmc1蛋白在進行DNA股交換時的核蛋白結構

Investigating Human Dmc1 Nucleoprotein Structure During DNA Strand Exchange Using Single Molecule Methods

呂易翰 , Masters　　Advisor：李弘文　　

繁體中文

同源重組； DNA 修復； Dmc1 ；單分子栓球實驗；自相關函數； homologous recombination ； DNA repair ； Dmc1 ； tethered particle motion ； autocorrelation

Abstract │ Reference (65) │ Altmetrics

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Cells utilize homologous recombination (HR) to repair the DNA double-strand break (DSB). The reaction is conducted by a kinds of protein called recombinase. While conducting the HR reaction, recombinase need to bind with ATP and then assemble on single-stranded (ss) DNA to form nucleoprotein complex. After the strand exchange completed, ATP was hydrolyzed and recombinase disassemble from DNA. Dmc1 is a meiosis-specific recombinase responsible for HR in eukaryotic cell. Unlike other two Dmc1 homolog RecA and Rad51, which have been proved to form an extended nucleoprotein filament, Dmc1 was showed to have two kinds of structure of nucleoprotein filament by electron microscopy studies, one is extended nucleoprotein filament and another is stacked ring structure. In this study we use tethered particle motion (TPM) to observe the real time behavior while Dmc1-catalyzed strand exchange reaction was processing and found that Dmc1 does not form an extended three strand intermediate, even though we add more calcium to inhibit ATP hydeolysis and stabilize the Dmc1 filament. So we further utilize the autocorrelation function to analyze the reaction timetrace and prove the DNA-protein interaction.

Reference ( 65 ) 〈TOP〉

1 Kleckner, N. Meiosis: how could it work? Proc. Natl. Acad. Sci. USA 93, 8167-8174 (1996).
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2 Cox, M. M. Recombinational DNA repair in bacteria and the RecA protein. Prog Nucleic Acid Res. Mol. Biol. 63, 311-366 (1999).
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3 Mao, Z., Bozzella, M., Seluanov, A. & Gorbunova, V. Comparison of nonhomologous end joining and homologous recombination in human cells. DNA Repair 7, 1765-1771 (2008).
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4 Inagaki, A., Schoenmakers, S. & Baarends, W. M. DNA double strand break repair, chromosome synapsis and transcriptional silencing in meiosis. Epigenetics 5, 255-266 (2010).
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5 Sung, P. & Klein, H. Mechanism of homologous recombination: mediators and helicases take on regulatory functions. Nat. Rev. Mol. Cell Biol. 7, 739-750 (2006).
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