靜磁場增強辣椒素對人類肝癌細胞的抗癌作用

長久以來，人類飽受癌症之苦，在台灣又以肝癌為大宗。而目前對於肝癌的診治，多數以手術或者化療等進行治療，然而這些治療方式為身體所帶來的副作用難以忽視，因此科學家逐漸將目光轉向較無副作用的天然食材。除了傳統藥物相關方法之外，使用物理場來進行治療也是近來新興起的領域，由於物理場對人體副作用低，所以物理場的治療法也漸漸被引入醫療領域中。綜合以上，將藥物或食材結合物理場來放大抑制效果，進而達到低劑量高成效更是一種值得研究的新方向。有鑑於此，我們決定研究台灣日常飲食中常見的食材-辣椒，以及其協同磁場共同抗癌的成效。辣椒是常見的食物，其所含的辣椒素為辣椒辣度來源的主要構成成分。根據過去的研究指出，一定濃度以上的辣椒素對於人類肝癌細胞能夠達到抑制的效果；而在物理場方面，隨著生物相關之磁效應的發現以及應用，使學界認為磁場確實會對生物組織造成一定程度的影響。本實驗旨在探討磁場協助辣椒素抑制肝癌的效果。實驗中選用HepG2細胞株來模擬人類肝癌細胞，在培養中的HepG2細胞加入辣椒素之後，置於強度0.5T的靜磁場裝置上，操作三天後透過光學顯微鏡觀察細胞狀態，接著使用MTT assay收取細胞存活率數據、透過Flow Cytometer測定細胞死亡狀態、西方墨點法收取BCL-2及BAX蛋白表現量，並於共軛焦螢光顯微鏡拍攝cleaved caspase-3的免疫螢光染色圖來觀察凋亡相關蛋白的表現。實驗數據顯示靜磁場協同辣椒素比起單獨加入辣椒素的組別，有更好的抑制肝癌細胞效果。而BAX與BCL-2細胞等凋亡相關蛋白的表現比例，以及免疫螢光染色圖中的cleaved caspase-3表現量都指出靜磁場協同辣椒素組別的細胞，比起單獨加入辣椒素的組別傾向凋亡的程度更為明顯。總結本實驗的結果，強度0.5T的靜磁場協同辣椒素，相較於單獨使用辣椒素能夠達到更高的抑制人類肝癌細胞的效果。

關鍵字

辣椒素；靜磁場；肝癌

並列摘要

Human suffer from cancer for a long time, and there are a large number of liver cancer patients in Taiwan. In the past, treatments for liver cancer patients are surgery or drug-based, however, the side effects for these treatments cannot be ignored. In order to change this situation, scientists target on natural ingredients which are relatively harmless. Apart from the traditional method by treating cancer with drugs, treatment by physical fields is a rising area in recent years. Due to its low side effects on the human body, physical field has gradually been introduced into the medical field as a therapy. By combining the noninvasive physical field with natural ingredients, the anticancer effect of natural compound could be enhanced, which allows minimum dosage of drugs to produce maximum curative effect. In view of this, we decided to study the daily diet in Taiwan - Capsicum annuum, as well as its synergistic anticancer effect with magnetic field. Capsicum annuum contains capsaicin, which is the main constituent accounting for its spiciness. Past studies have pointed out that above a certain concentration, capsaicin could induce apoptosis in HepG2. In terms of physical fields, with the discoveries and applications of the bio-magnetic effects, scholars believe that biological tissue would indeed affect by magnetic field. This study was designed to investigate the magnetic field-assisted inhibiting effect of capsaicin in liver cancer. We use HepG2 cell line as a model system for human liver cancer cells. In the culture of HepG2 cells, we added capsaicin and placed under a static magnetic field (0.5T). Three days after operation, the state of cells was observed by an optical microscope. MTT assay was used to detect cell viability. Cell death status was measured by Flow Cytometry. Expression of apoptosis-related proteins BCL-2 and BAX was measured by Western Blot, and cleaved caspase-3 protein was detected via immunofluorescence staining and photographed through a confocal fluorescence microscopy. Experimental data showed that compared to capsaicin alone group, capsaicin synergized with static magnetic field (0.5T), which had a better effect on inhibiting HepG2. The expression ratio of BAX to BCL-2 and cleaved caspase-3 also pointed out that capsaicin work synergistically with static magnetic field causing apoptosis effects more obvious than using capsaicin alone on HepG2. In summary, our results show that static magnetic field enhanced the anticancer effect of capsaicin on HepG2.

並列關鍵字

Capsaicin ； Static Magnetic Field ； HepG2

參考文獻

28 Abdel-Salam, O. M. Capsaicin as a Therapeutic Molecule. (Springer, 2014).

1 Davies, A. M., Weinberg, U. & Palti, Y. Tumor treating fields: a new frontier in cancer therapy. Ann N Y Acad Sci 1291, 86-95, doi:10.1111/nyas.12112 (2013).

2 Ren, Z. et al. Nanosecond pulsed electric field inhibits cancer growth followed by alteration in expressions of NF-kappaB and Wnt/beta-catenin signaling molecules. PLoS One 8, e74322, doi:10.1371/journal.pone.0074322 (2013).

3 Wang, J. et al. Synergistic effects of nanosecond pulsed electric fields combined with low concentration of gemcitabine on human oral squamous cell carcinoma in vitro. PLoS One 7, e43213, doi:10.1371/journal.pone.0043213 (2012).

4 Taunton, J. How to starve a tumor. Chemistry & biology 4, 493-496 (1997).

國際替代計量

靜磁場增強辣椒素對人類肝癌細胞的抗癌作用

全文下載

主題瀏覽