於改良式漸縮微流道拉伸DNA之研究

厲承翰

doi:10.6342/NTU.2012.01832

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於改良式漸縮微流道拉伸DNA之研究

Research of DNA stretching in a modified microcontraction

厲承翰 , Masters　　Advisor：謝之真　　

繁體中文 DOI： 10.6342/NTU.2012.01832

拉伸DNA ；漸縮微流道；壓力流；電泳； DNA stretching ； microcontraction ； pressure-driven flow ； electrophoresis

Abstract │ Reference (42) │ Times Cited (1) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

We experimentally test two microfluidic devices for stretching DNA using electric field and flow field. The microfluidic devices were designed with the help of computer simulations for electrophoretic stretching of DNA, and both were predicted to outperform a simple contraction. We first electrophoretically stretch DNA using DC field while the experimental results were not even in qualitative agreement with our simulations. Detailed investigation in experiments reveals that DNA adapts a compressed conformation in high DC field and thus becomes more difficult to stretch. 
Due to the similarity between flow field and electric field, we use the same microfluidic devices with flow field to stretch DNA. The experimentally observed DNA behavior in flow field is similar to the prediction based on electric field. The rotation-extension motion of DNA that is the key to the preconditioning effect is also observed in flow field. As to the performance for DNA stretching, case III is better than case II, and case II is better than case I. The result is again consistent with our prediction.

Reference ( 42 ) 〈TOP〉

1. Hsieh, C.C., Simulation of conformational preconditioning strategies for electrophoretic stretching of DNA in a microcontraction. Biomicrofluidics, 2011. 5: p. 044106.
連結：
2. Randall, G.C., K.M. Schultz, and P.S. Doyle, Methods to electrophoretically stretch DNA: microcontractions, gels, and hybrid gel-microcontraction devices. Lab on a Chip, 2006. 6(4): p. 516-525.
連結：
3. Chan, E.Y., et al., DNA mapping using microfluidic stretching and single-molecule detection of fluorescent site-specific tags. Genome Research, 2004. 14(6): p. 1137-1146.
連結：
5. Zimm, B.H., Extension in flow of a DNA molecule tethered at one end. Macromolecules, 1998. 31(18): p. 6089-6098.
連結：
6. Randall, G.C. and P.S. Doyle, DNA deformation in electric fields: DNA driven past a cylindrical obstruction. Macromolecules, 2005. 38(6): p. 2410-2418.
連結：

Times Cited (1) 〈TOP〉

劉貞汝(2017)。結合圓柱陣列與漸擴微流道以電泳分離DNA之研究。臺灣大學化學工程學研究所學位論文。2017。1-122。

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