利用半胱胺酸蛋白酶抑制因子及幾丁質酶之雙基因轉殖發展抗根瘤線蟲的耐熱番茄品系

蕭岑慈

doi:10.6342/NTU.2010.01749

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利用半胱胺酸蛋白酶抑制因子及幾丁質酶之雙基因轉殖發展抗根瘤線蟲的耐熱番茄品系

Engineering the heat-tolerant tomato plant with a stacking of cysteine protease inhibitor and chitinase genes exhibits resistance against root-knot nematode (Meloidogyne incognita)

蕭岑慈 , Masters　　Advisor：葉開溫　　

繁體中文 DOI： 10.6342/NTU.2010.01749

南方根瘤線蟲；半胱胺酸抑制因子；幾丁質酶； root-knot nematode ； Colocasia esculenta cysteine protease inhibitor (CeCPI) ； Paecilomyces javanicus-1 (PjCHI-1)

Abstract │ Reference (76) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Root-knot nematodes are sendentary parasitic nematode, they attack multiple economic crops and cause productivity and economic losses. The strategies of nematode control are culture management, resistant cultivar and nematocide. Although useful nematocide is used to reduce nematode population, it causes the enormous harm to human and environment. Through, our goal is to engineer the nematode-resistant plant to replace nematocide. We construct the Colocasia esculenta cysteine protease inhibitor (CeCPI) and Paecilomyces javanicus chitinase-1(PjCHI-1) driven by a synthetic promoter, pMSPOA . 6 weeks afeter root-knot nematode infection, we calculate the root galls and egg mass, and we find that the number of root galls and egg mass are reduced. In the previous study, we know that CeCPI can affect the nematode digestion and PjCHI-1 can destroy the egg shell. The transgenic plants showed prominently the reduction of saccate, decline of enlarged saccate female percentage, lack of chitin content, decrease of eggs per egg mass and retardation of embryogenesis. In conclusion, the transgenic plants which are overexpressed PjCHI nad CeCPI can effectively control the propagation Meloidogyne incognita and to decline nematode progeny.

Reference ( 76 ) 〈TOP〉

陳正次 (2002) 番茄病蟲害與生理障礙的防治。番茄品種特性與栽培技術全輯。行政院農委會種苗改良繁殖場。台灣。P56-78。
連結：
張孝齊 (2005) 甘藷 sporamin 基因啟動子上順向作用的 DNA 片段之功能性分析及 sporamin 基因家族成員間變異性之研究。國立台灣大學植物科學研究所碩士論文。
連結：
楊藹華 (2004) 芋頭半胱胺酸蛋白酶抑制劑 (Tarocystatin) 之基因選殖、表現特性及對微生物抗性的分子基至研究。國立台灣大學植物科學研究所博士論文。
連結：
蔡東纂 (1996) 台灣作物線蟲病連作帳害之發生及對策。植病會刊 5: 113-128.
連結：
Adams, D. J. (2004) Fungal cell wall chitinase and glucanases. Microbiology 150: 2029-35.
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