結合蜂蜜花粉分析與高效液相層析鑑定台灣荔枝蜜與龍眼蜜

楊富鈞

doi:10.6342/NTU.2012.02140

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結合蜂蜜花粉分析與高效液相層析鑑定台灣荔枝蜜與龍眼蜜

Combining melissopalynological analysis and high performance liquid chromatography for the identification of litchi- and longan-honeys in Taiwan

楊富鈞 , Masters　　Advisor：陳淑華　　Co-advisor ：吳俊宗

繁體中文 DOI： 10.6342/NTU.2012.02140

龍眼蜜；荔枝蜜；蜂蜜花粉分析；高效液相層析；植物源標記； litchi honey ； longan honey ； melissopalynology ； HPLC ； botanical marker

Abstract │ Reference (136) │ Times Cited (1) │ Altmetrics

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Litchi (Litchi chinensis) and longan (Euphoria longan) are two of most important nectariferous plants for monofloral honeys in Taiwan. Owing to high similarity in pollen morphology and overlapping of flowering time in some areas, it is difficult to distinguish either kind of honey. In order to set up a method for identifying them, we collected in this study 43 litchi honeys, 94 longan honeys, 10 mixtures of litchi- and longan-honeys, 28 other honeys from Taiwan and 3 foreign lhoneys from foreign country over the time from March to May, 2011. Melissopalynological analysis was applied to construct the pollen profiles. In addition, samples were extracted with solid-phase extraction, followed by high performance liquid chromatography (HPLC) detected at 280 nm to find out reliable botanical markers. In studied samples, there were 98 pollen types belonging to 51 families. The frequencies of the litchi-longan type pollen grains were generally above 50% in litch- and longan-honeys and were readily differentiated from other Taiwan honeys.. Both litchi- and longan-honeys displayed three characteristic peaks (A, B, D) in HPLC-chromatogram. Semi-quantitative analysis showed that the peak D was significantly lower for litchi honeys, whereas higher for longan honeys. The results of melissopalynological and HPLC analyses supported that A, B and D peaks originated mainly from litchi– and longan-nectars, and could be used as the botanical markers of ether type of honeys. It is suggested those labeled with typical litchi- or longan-honey should have pollen frequency higher than 50%, respectively.

Reference ( 136 ) 〈TOP〉

林秋惠（2007）。台灣欒樹雄花及兩性花花部發育與小孢子形成之研究。台灣大學生態學與演化生物學研究所碩士論文。
連結：
林庭妤（2010）。台灣蜂蜜抗氧化活性分析及抗菌特性之研究。虎尾科技大學光電與材料科技研究所碩士論文。
連結：
陳宜君與區少梅（2008）。以近紅外線光譜技術鑑別台灣真假蜂蜜。台灣農業化學與食品科學，第46卷，第129至137頁。
連結：
陳淑華（2000）。國產與進口龍眼蜜之鑑定。農委會中部辦公室行政及建設計畫成果報告。
連結：
陳翠華（2008）。台灣市售蜂蜜消費行為及喜好性調查。中興大學食品暨應用生物科技學系碩士論文。
連結：

Times Cited (1) 〈TOP〉

吳宗元(2015)。直接大氣壓下化學游離質譜法應用於蜂蜜不同蜜源及產地之鑑定。虎尾科技大學生物科技研究所學位論文。2015。1-93。

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