利用豬關節軟骨碎片混合富血小板纖維素進行豬隻自體關節軟骨組織工程再生

包庸宣

doi:10.6342/NTU.2009.02783

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利用豬關節軟骨碎片混合富血小板纖維素進行豬隻自體關節軟骨組織工程再生

Use of Platelet-rich Fibrin (PRF) as Scaffold Seeded with Porcine Articular Cartilage Fragments for Tissue Engineering: an in vivo Autologous Transplantation Analysis

包庸宣 , Masters　　Advisor：郭宗甫　　

英文 DOI： 10.6342/NTU.2009.02783

豬；關節軟骨；富血小板纖維素；組織工程； porcine ； cartilage ； platelet-rich fibrin (PRF) ； tissue engineering

Abstract │ Reference (95) │ Times Cited (1) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Introduction Adult articular cartilage is characterized by a poor ability to repair damage spontaneously. In the study of tissue engineering, scaffold provides a physicochemical and biological three－dimensional microenvironment for cell growth. Platelet rich fibrin   (PRF) was a second-generation platelet concentrate that has a gel-like construct with plenty of growth factors. It composed of fibrin fibrilla, fibronectin, glycanic chain, and platelet to form a network structure. 
Object Since most studies reported by means of transplantation, the aim of our study could be achieved by shifting this autologous cartilage shreds quickly during open articular surgery procedure. 
Experimental design, There were three experimental groups and control group (n=4), were established in our study. Experimental groups were separated into PRF group (implants with platelet-rich fibrin, n=4), CAR group (implants with cartilage fragments, n=4), and P/C group (cartilage fragments embedded with platelet-rich fibrin, n=4). The articular cartilages harvested from 16 Lan Yu pigs after implantation for 6 months.
Result To inspect the defect margin of native and regenerated cartilage, the edge is almost invisible in P/C group and approximately visible present in control group. There are significantly different in the parameters of coverage color and defect margin (p>0.05). Matrix stiffness test is ranked as normal cartilage>experimental groups>control group; exactly, the stiffness test between three experimental groups are ranked as and was ranked as P/C group>PRF groups>CAR group (p<0.05). Under histological assessment, the gap of P/C group lefts only point historical remains and most of the repair cartilage is fusion with native cartilage. Besides, the surface of P/C group regenerates with mostly smooth surface, and result also equal to the gross appearance. The matrixes of P/C group augment with large partial of hyaline-like cartilage and the macromolecular of matrix displays homogenously under Alcian blue and Safranin O staining. Regeneration of P/C group under histological analysis was agreement to the cartilage compressive stiffness test. The repair matrix of P/C group was mostly near to native cartilage that could predict the well mechanical function in prognosis.
Conclusion In summary, platelet-rich fibrin was able to help chondrocytes regeneration and cartilage fragments were proficient to deliver cartilage matrix and chondrocytes. Because of suitable tissue scaffold and cartilage fragments, our results suggested that the tissue regeneration of P/C group was facility to regenerate cartilage micro and macro-structure, the mechanical function and weight-bearing restored nearly to the native cartilage.

Reference ( 95 ) 〈TOP〉

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Times Cited (1) 〈TOP〉

曾嘉川(2014)。以可注射性富含血小板纖維素釋放液混合骨髓幹細胞治療兔膝關節軟骨再生之研究。臺灣大學獸醫學研究所學位論文。2014。1-69。

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