HER-2 siRNA造成HER-2大量表現癌細胞凋亡之研究

李幸芳

doi:10.6342/NTU.2004.01750

18.204.42.98，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

Health & Medical Care > Basic Medical Science
College of Medicine > Graduate Institute of Microbiology

Bibliography Management Tool

Related Links

Report an Issue

Purchase Single Articles

Download PDF

HER-2 siRNA造成HER-2大量表現癌細胞凋亡之研究

Induction of Apoptosis in HER-2-overexpressing Cancer Cells by siRNA against HER-2

李幸芳 , Masters　　Advisor：王萬波　　

繁體中文 DOI： 10.6342/NTU.2004.01750

表皮生長因子接受器；小干擾核醣核酸； HER-2 ； siRNA

Abstract │ Reference (44) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

HER-2 gene is a proto-oncogene which belongs to the epidermal growth factor receptor family. It is overexpressed in many human cancers, including breast, lung, ovary, pancreas, gastric, and oral cancers. HER-2-overexpressing cancer cells usually have higher potential to proliferate, metastasize, induce angiogenesis, and resist chemotherapeutic agents. Inhibition of HER-2 can lead to suppression of the tumorigenicity of HER-2-overexpressing cancer cells. Therefore HER-2/neu gene is a suitable target for cancer treatment.
To search for new ways to treat HER-2-overexpressing cancers, I have used small interfering RNA (siRNA) strategy to inhibit HER-2 expression in HER-2-overexpressing cancer cells. Three pSUPER plasmids which express different HER-2 siRNA were constructed. These plasmids were tested for their ability to inhibit HER-2 expression and to induce apoptosis of HER-2-overexpressing cancer cells. I have found that one of these siRNA constructs, HER-2-siRNA-3, can efficiently inhibit HER-2 expression in HER-2–overexpressing cancer cells. Moreover, this siRNA construct was shown to be able to specifically induce apoptosis in HER-2-overexpressing cancer cells but not in HER-2 low-expressing cancer cells. I also constructed a recombinant adenovirus, rAd-siHER-2-3, that can expresses HER-2-siRNA-3. rAd-siHER-2-3 infection was shown to be able to inhibit HER-2 expression and induce apoptosis in HER-2-overexpressing cancer cells. Taken together, these data suggest that HER2-siRNA-3 may be developed as a therapeutic agent against HER-2-overexpressing cancers.

Reference ( 44 ) 〈TOP〉

Adlkofer, K. & Lai, C. (2000). Role of neuregulins in glial cell development. Glia 29, 104-111.
連結：
Boilly, B., Vercoutter-Edouart, A. S., Hondermarck, H., Nurcombe, V. & Le Bourhis, X. (2000). FGF signals for cell proliferation and migration through different pathways. Cytokine Growth Factor Rev 11, 295-302.
連結：
Britsch, S., Li, L., Kirchhoff, S., Theuring, F., Brinkmann, V., Birchmeier, C. & Riethmacher, D. (1998). The ErbB2 and ErbB3 receptors and their ligand, neuregulin-1, are essential for development of the sympathetic nervous system. Genes Dev 12, 1825-1836.
連結：
Brummelkamp, T. R., Bernards, R. & Agami, R. (2002). A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550-553.
連結：
Chen, H., Yu, D., Chinnadurai, G., Karunagaran, D. & Hung, M. C. (1997). Mapping of adenovirus 5 E1A domains responsible for suppression of neu-mediated transformation via transcriptional repression of neu. Oncogene 14, 1965-1971.
連結：

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：