靈芝屬菌株錳過氧化酶的選殖與表達

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靈芝屬菌株錳過氧化酶的選殖與表達

Cloning and Expression of Manganese Peroxidase Genes from Ganoderma spp.

賴志穎 , Masters　　Advisor：許瑞祥　　

繁體中文

錳過氧化酶靈芝；白腐型真菌；畢赤氏酵母；木質素； Manganese peroxidase ； MnP ； Ganoderma ； White rot fungi ； Pichia methanolica ； lignin

Abstract │ Reference (139) │ Times Cited (1) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Lignin, component of plant secondary cell wall, binds to hemicellulose by covalent bonds and defends bacteria or fungi invading into the core of the wood. Lignin is a complicated polymer composed of aromatic compounds, which are ferulic acid, sinapic acid and p-coumaric acid etc. In paper pulping industry, lignin is hard-degradable and influences the brightness of paper. Generally, people use concentrated acid or alkali to precipitate or dissolve lignin. In recent study, the paper would be brighter and whiter by using manganese peroxidase in pulping procedure.
  Manganese peroxidase, which was first isolated from white rot fungi Phanerochaete chrysosporium by Glenn and Gold in 1985, belongs to heme-containing peroxidase family. This extracellular enzyme is a glycoprotein with five disulfide bonds in general and contains two calcium ions in the structure. When it catalyses the substrates, hydrogen peroxide which produced by glyoxal oxidase or aryl-alcohol oxidase is necessary as electron donor. Mn(II) would be oxidized to Mn(III) by heme-oxygen radical complex. Mn(III) cations would be stabilized by alfa-hydroxyl acids, such as succinic acid or lactic acid, and oxidize lignin or lignin-like compounds. 
  Manganese peroxidase has been found in many of white rot fungi, including Trametes versicolor, Pleurotus ostreatus, Armillaria mellea, etc, however, the studies of manganese peroxidase from Ganoderma spp. are few and most of them were focus on preotein level. The only published cDNA sequence was from G. applanatum. In this study, we cloned six new sequences of manganese peroxidase from G. formosanum, G. neo-japonicum and G. australe. Furthermore, we expressed the manganese peroxidase of G. formosanum in Escherichia coli and Pichia methanolica and produced recombinant enzyme.

Reference ( 139 ) 〈TOP〉

3. Aggelis, G., C. Ehaliotis, F. Nerud, I. Stoychev, G. Lyberatos, and G. I. Zervakis. 2002. Evaluation of white-rot fungi for detoxification and decolorization of effluents from the green olive debittering process. Appl Microbiol Biotechnol 59:353-60.
連結：
4. Ahuja, S. K., G. M. Ferreira, and A. R. Moreira. 2004. Utilization of enzymes for environmental applications. Crit Rev Biotechnol 24:125-54.
連結：
6. Al-Samarrai, T. H., and J. Schmid. 2000. A simple method for extraction of fungal genomic DNA. Lett Appl Microbiol 30:53-6.
連結：
7. Austin, S., E. T. Bingham, D. E. Mathews, M. N. Shahan, J. Will, and R. R. Burgess. 1995. Production and field performance of transgenic alfalfa (Medicago sativa L.) expression alpha-amylase and manganese-dependent lignin peroxidase. Euphytica 85:381-93.
連結：
8. Bai, Z., L. M. Harvey, and B. McNeil. 2003. Oxidative stress in submerged cultures of fungi. Crit Rev Biotechnol 23:267-302.
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Times Cited (1) 〈TOP〉

陳明煦(2006)。台灣紫芝生物活性代謝產物之醱酵生產與功能評估。臺灣大學微生物與生化學研究所學位論文。2006。1-118。

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