配體修飾之量子點應用於細胞表面分子動態變化之研究

蔡幸珊

doi:10.6342/NTU.2008.02652

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配體修飾之量子點應用於細胞表面分子動態變化之研究

Study of Membrane Molecules Dynamics Using Ligand-Modified Quantum Dots in Optical Imaging

蔡幸珊 , Masters　　Advisor：張富雄　　

繁體中文 DOI： 10.6342/NTU.2008.02652

量子點；單一分子追蹤；刀豆凝集素；小麥凝集素；抗細胞黏附因子抗體α1,β1 ；三倍頻顯微影像系統； Quantum dots ； Single molecule tracking ； ConA ； WGA ； Anti-integrin αv, β1 ； Third harmonic generation imaging system

Abstract │ Reference (40) │ Altmetrics

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According to the progress in the nanotechnology and opto-electronics imaging, the scientists integrate nanoparticles with microscope imaging system and apply to molecular imaging in the biomedicine. Quantum dot is a familiar nanoparticle which has a lot of unique optical properties. Therefore, quantum dot has emerged as a new fluorescent probe for biomolecular imaging.
    Quantum dot has better photostability and less photobleach in long term laser excitation than conventional dyes. Base on this, we can combine quantum dots with ultrasensitive optical technology to provide the high signal-to-noise ratio and allow for long term observation. In order to understand the dynamics of temporal and spatial at molecular scale, we can track the motions of the single molecule, record the trajectory, and even investigate the intermolecular interaction by optical technology.
    In this research, we use lectins (ConA, WGA) and antibody (anti-integrin αv, β1) to recognize the surface makers on the mouse or human neuroblastoma cells (Neuro2A and SH-SY5Y) to track the dynamics of the specific molecule. In the experiments, we use natural lipids (DPPC/Cholesterol/DSPE-PEG/DOPE-biotin) coated quantum dots which is not only reduce the cytotoxity but also diminish the non-specific binding on the cell membrane. Due to quantum dot has fluorescence signal and lipid-coated quantum dots has third harmonic generation signal result from the difference of the interface. Therefore, we can observe the dynamic changes of the targeted molecule in live cells by fluorescent microscopy and third harmonic generation imaging system.
    In the future, applications of lipid-coated quantum dots combine optical imaging system in live cell imaging can investigate the responses between single receptor and drugs or the intermolecular interactions, and record the dynamic changes at the same time.

Reference ( 40 ) 〈TOP〉

[1] O. Salata, Applications of nanoparticles in biology and medicine. J Nanobiotechnology. 2 (2004) 1-6.
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[2] S. Nie, Y. Xing, G.J. Kim, J.W. Simons, Nanotechnology applications in cancer. Annu Rev Biomed Eng. 9 (2007) 257-288.
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[3] M. Bruchez, Jr., M. Moronne, P. Gin, S. Weiss, A.P. Alivisatos, Semiconductor nanocrystals as fluorescent biological labels. Science. 281 (1998) 2013-2016.
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[4] W.C. Chan, S. Nie, Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science. 281 (1998) 2016-2018.
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[5] P.F. Michalet X, Bentolila LA, Tsay JM, Doose S, Li JJ, et al., Quantum dots for live cells, in vivo imaging, and diagnostics. Science. 307 (2005) 538-544.
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