Luminol-Co(II)-NH2OH系統之雙重化學發光探討與其在分析上的應用

游信凱

doi:10.6342/NTU.2010.03285

3.236.75.30，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

Basic & Applied Sciences > Chemistry
College of Science > Graduate Institute of Chemistry

Bibliography Management Tool

Related Links

Report an Issue

Purchase Single Articles

Download PDF

Luminol-Co(II)-NH2OH系統之雙重化學發光探討與其在分析上的應用

Dual Chemiluminescence of luminol-Co(II)-NH2OH System and its Applications

游信凱 , Masters　　Advisor：林萬寅　　

繁體中文 DOI： 10.6342/NTU.2010.03285

化學發光；自由基消滅劑；停止流儀；酚類化合物；鈷離子； luminol ； chemiluminescence ； Co(II) ； free radical scanvenger ； phenolic compounds ； stopped flow spectrometry

Abstract │ Reference (35) │ Altmetrics

Parallel Abstract 〈TOP〉

Chemiluminescence (CL) is produced by the exothermic reaction. This study focused on the dual chemiluminescence of luminol-Co(II)-NH2OH. We found that concentrations of Co(II), EDTA, NH2OH and pH could lead to dual chemiluminescence. To know more about it, we’ve tried many conditions to construct the luminol-Co(II)-NH2OH system.
The results from stopped flow experiments showed that low concentrations of Co(II)(less than 3 uM) produced only one peak. At higher concentrations of Co(II) dual chemiluminescence appeared. Addtion of EDTA caused suppression and delayed of dual CL, suggesting the importance of free Co(II). Moreover, concentration of NH2OH and pH values between 12.5 and 13.5 were also required for the observation of dual CL.
  In order to know the mechanism of the dual chemiluminescence of luminol-Co(II)-NH2OH system, we tested a series of free radical scanvengers for their effect on CL emission. In the present of 1O2 scanvengers like NaN3, 1,4-diazabicyclo[2.2.2]octane and DMF, dual CL emission was enhanced. Addition of  ˙OH scanvengers such as CH3OH , DMSO and uric acid, inhibited peak 2 of dual CL. Captopril, a ˙O2- scanvenger, could inhibit and delay CL peaks. We concluded that   ˙O2- is involved in the CL emission of peak 1, while ˙O2- and ˙OH play key role for the second CL peak.
  By using the dual chemiluminescence of luminol-Co(II)-NH2OH system, we tested 58 compounds including amino acids, vitamins, antioxidants, reducing compounds and drugs for their effect on the CL emission. We found that phenolic compounds showed inhibiting effect on our CL system, especially benzenediol compounds, flavonoids and polyphenolic compounds. It was found that compounds bearing a dihydroxybenzene group, exhibited best inhibition on peak 1. Previous studies have suggested that dihydroxybenzenes were good superoxide anion radical scanvenger, causing CL inhibition. We proposed that superoxide anion radical plays a critical role in both CL peaks. The results suggest that this system could be used to screen the scanvenge activities and antioxidant activities of the compounds. Using the selectivity from inhibitng either peak 1 or peak 2, we could make use of the dual chemiluminescence of luminol-Co(II)-NH2OH for the analytic detection.

Reference ( 35 ) 〈TOP〉

1. Greer, L F & Szalay A A Luminescence 2002, 17, 43
連結：
2. Campbell A K Chemiluminescence: Principles and Applications in Biology and Medicine. New York, 1988, 19
連結：
3. Palilis L P & Calokerinos A C Anal. Chim. Acta 2000, 413, 175
連結：
4. Marquette C A & Blum L J Anal Bioanal Chem 2006, 385, 546
連結：
5. Evmiridis N P, Vlessidis A G, Thanasoulias N C Bioinorg Chem Appl 2007, 92595.
連結：

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：