- 學位論文
蜂膠對單純疱疹病毒及小兒麻痺病毒之藥物感受性試驗
Propolis Susceptibility Testing of Herpes Simplex Virus and Poliovirus
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摘要
本研究主要針對蜂膠 (propolis)抑制病毒活性效果做一初步之探討。由以往研究知道，蜂膠對一些病毒如第一型單純疱疹病毒及鼻病毒等有抑制作用，為瞭解蜂膠對第二型單純疱疹病毒，及與鼻病毒屬同一科之腸病毒是否有抑制作用，因此進行實驗。將來自台灣與法國產地之蜂膠以水或酒精萃取後，分別以in vitro分析方式進行測試。結果顯示，0.5％水溶液萃取物及0.1％酒精溶液萃取物均可以抑制100TCID50 (Tissue Culture Infectious Dose50; 50% 培養組織受病毒感染之劑量) 劑量的第一型、第二型單純疱疹病毒；但兩者對第一型、第二型、及第三型小兒麻痺病毒均無抑制作用。以0.5％ 之水溶液萃取物分別與病毒作用1、2、4、16小時後發現，在2小時即可降低病毒活性；而不具套膜的小兒麻痺病毒即使經由最高劑量，即1% 蜂膠酒精萃取物，處理16小時後，仍無明顯的抑制效果。除台灣產的蜂膠外，本研究亦分析來自法國之蜂膠。結果顯示，法國產之0.1%蜂膠水溶液萃取物有顯著抑制單純疱疹病毒的效果；而台灣產的0.1%蜂膠水溶液萃取物則沒有明顯的抑制效果，需0.5% 才能抑制單純疱疹病毒。有關蜂膠抑制單純疱疹病毒的機制仍有待進一步探討。
並列摘要
The object of this study is focused on the inhibitory effect of proplis on viral infectivity. It has been reported that propolis possessed inhibitory effects on herpes simplex virus type 1 (HSV-1) and rhinovirus. In this report, the HSV-2 and enteroviruses which belong to the same family of picornviruses as rhinovirus were used as the subjects to evaluate the inhibitory effect of propolis. The propolis from Taiwan or France were extracted with water or ethanol and used for subsequent in vitro study. The results showed that the minimal inhibitory concentration for HSV-1 and HSV-2 were 0.5% for water extracted propolis and 0.1% for ethanol extracted propolis. No inhibitory effect on poliovirus type 1, poliovirus type 2 or poliovirus type 3 were observed. The results of timing study revealed that the maximal inhibitory effect on HSV-1 and HSV-2 were found at 2 hours after propolis application.The non-lipid-enveloped virus, enterovirus, has no response to propolis treatment even at the 1% concentration of ethanol extracted propolis. In addition, at 0.1% concentration, propolis from Frence has distinct inhibitory effect to HSV-1. Propolis from Taiwan only showed inhibitory effect at 0.5% concentration. The mechanisms of this inhibitory effect to HSV need to be further studied in the future.
參考文獻
1. Santos, F.A., et al., Brazilian propolis: physicochemical properties, plant origin and antibacterial activity on periodontopathogens. Phytother Res, 2003. 17(3): p. 285-9.
2. Pietta, P.G., C. Gardana, and A.M. Pietta, Analytical methods for quality control of propolis. Fitoterapia, 2002. 73 Suppl 1: p. S7-20.
3. Velikova, M., et al., Propolis from the Mediterranean region: chemical composition and antimicrobial activity. Z Naturforsch [C], 2000. 55(9-10): p. 790-3.
5. Khayyal, M.T., et al., A clinical pharmacological study of the potential beneficial effects of a propolis food product as an adjuvant in asthmatic patients. Fundam Clin Pharmacol, 2003. 17(1): p. 93-102.
6. Grange, J.M. and R.W. Davey, Antibacterial properties of propolis (bee glue). J R Soc Med, 1990. 83(3): p. 159-60.