酵母菌YPL014W蛋白之功能特徵分析

王語瑄

doi:10.6342/NTU.2006.03123

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酵母菌YPL014W蛋白之功能特徵分析

Functional characterization of yeast YPL014W

王語瑄 , Masters　　Advisor：鄧述諄　　

繁體中文 DOI： 10.6342/NTU.2006.03123

YPL014W ； YPL014W

Abstract │ Reference (41) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Telomere is replicated by telomerase in most eucaryotes. Most yeast cells that lack telomerase enter into senescence and eventually die. However, a few  cells bypass the senescence phenotype and survive. These survivors elongate their telomeres by the alternative recombination pathway. The transcriptional level of YPL014W is increased in telomerase-independent alternative lengthening of telomeres (ALT) pathway, which is used by type II survivor cells. Previous study showed that Ypl014w forms a complex with Cdc28 and Cln3(Uetz et al., 2000), indicating that Ypl014w may involve in cell cycle regulation. Study the function of Ypl014w may help us to find the relationship between telomere elongation pathway and cell cycle regulation.
    In my study, YPL014W knockout has no influence on cell cycle regulation and the phosphorylation status of Cdc28-Cln3 substrate Whi5. I also find that Ypl014w is a phosphorylated protein, but CLN3 knockout and temperature sensitive mutant of CDC28 have no influence on its phosphorylation status. I did not find the redundant gene of YPL014W with synthetic lethal. I can not confirm the previous study that Ypl014w interacts with Cdc28 and Cln3 in yeast two hybrid. I did not find other proteins that interact with Ypl014w in yeast-two-hybrid screen and IP analysis.
    According to sequence analysis, YPL014W expression may be controlled by Mcm1, a transcription factor that regulates many cell cycle regulated genes. I showed that Mcm1 binds to the promoter of YPL014W with CHIP and found the two ECB elements that Mcm1 binds.

Reference ( 41 ) 〈TOP〉

Abraham, D. S., and Vershon, A. K. (2005). N-terminal arm of Mcm1 is required for transcription of a subset of genes involved in maintenance of the cell wall. Eukaryot Cell 4, 1808-1819.
連結：
Chang, V. K., Donato, J. J., Chan, C. S., and Tye, B. K. (2004). Mcm1 promotes replication initiation by binding specific elements at replication origins. Mol Cell Biol 24, 6514-6524.
連結：
Chen, Q., Ijpma, A., and Greider, C. W. (2001). Two survivor pathways that allow growth in the absence of telomerase are generated by distinct telomere recombination events. Mol Cell Biol 21, 1819-1827.
連結：
Costanzo, M., Nishikawa, J. L., Tang, X., Millman, J. S., Schub, O., Breitkreuz, K., Dewar, D., Rupes, I., Andrews, B., and Tyers, M. (2004). CDK activity antagonizes Whi5, an inhibitor of G1/S transcription in yeast. Cell 117, 899-913.
連結：
Costanzo, M., Schub, O., and Andrews, B. (2003). G1 transcription factors are differentially regulated in Saccharomyces cerevisiae by the Swi6-binding protein Stb1. Mol Cell Biol 23, 5064-5077.
連結：

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