幾丁聚醣及其酵素水解物對細菌及果蠅幼蟲之抑制活性

Inhibitory activities of chitosan and its enzymatic hydrolysates against bacteria and Drosophila larval

林政毅(Cheng-Yi Lin)

指導教授 ： 王三郎

淡江大學/理學院/化學學系碩士班/碩士(2011年)

https://doi.org/10.6846/TKU.2011.01019

摘要

本研究係以Bacillus cereus TKU006、Serratia ureilytica TKU013、Pseudomonas sp.TKU015三株幾丁質酶/幾丁聚醣酶生產菌發酵製備出粗酵素液。以酵素法水解懸浮態幾丁質、水溶性幾丁聚醣，製備不同水解時間之酵素水解物並以其進行抗菌及抗果蠅實驗，探討酵素水解物之對E.coli、B.subtilis及果蠅幼蟲之抑制活性。
抗E.coli測試中，WSC酵素水解物依水解程度不同，對E.coli與B.subtilis在抗菌效果上有顯著的不同抑制效果，樣品WSC/013/1h有最佳抑制效果，IC50為0.1%。抗B.subtilis測試中，樣品WSC/015/12h有最佳抑制效果，IC50在0.07%以下。
在果蠅幼蟲毒殺實驗，酵素水解懸浮態幾丁質之水解物中，CC/015水解物以水解第1小時之樣品對果蠅有最佳毒殺效果，致死率達87.5%。

關鍵字

幾丁聚醣 ； 幾丁質酶 ； 幾丁聚醣酶 ； 酵素水解物 ； 抗菌 ； 果蠅幼蟲

並列摘要

Preparation of crude enzyme solutions from three chitinase/chito- sanase-produced strains, Bacillus cereus TKU006, Serratia ureilytica TKU013, and Pseudomonas sp.TKU015 to hydrolyze prepared from colloidal chitin(CC) and water soluable chitosan(WSC).
Hydrolysates were collected at several points in time. The bio- activity of these hydrolysates against bacteria, Drosophila larval were studied.
The inhibitory activity has a significant difference between against E.coli and B.subtilis treating with different hydrolysates. WSC hydrolysates prepared using TKU013 enzyme at hydrolysis time of 1h which had better antibacterial activity against E.coli, IC50=0.1%. WSC/015/12h had better antibacterial activity against B.subtilis, IC50<0.07%.
CC/015/0h used in anti-Drosophila activity tests shows best insecticidal effect(morality=87.5%).

並列關鍵字

chitosan ； chitinase ； chitosanase ； enzymatic hydrolysate ； antibacterial ； Drosophila larval

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