血清學活動臨床穩定全身性紅斑性狼瘡探討影響吞噬作用及清除凋亡細胞之因素

黃文男

doi:10.6834/CSMU.2012.00024

44.197.230.180，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

Health & Medical Care > Medical Sciences
College of Medicine > Graduate Institute of Medicine

Bibliography Management Tool

Related Links

Report an Issue

Purchase Single Articles

Download PDF

血清學活動臨床穩定全身性紅斑性狼瘡探討影響吞噬作用及清除凋亡細胞之因素

The study of pathogenesis of Serology Active Clinically Quiescent Patients with Systemic Lupus Erythematosus in affecting phagocytosis and clearance of apoptotic cells

黃文男 , Ph.D　　Advisor：蔡嘉哲　　

繁體中文 DOI： 10.6834/CSMU.2012.00024

血清學活動臨床穩定全身性紅斑性狼瘡；凋亡細胞；巨噬細胞；第二型轉麩胺酶； Systemic lupus erythematousus ； serologically active clinically quiescent ； phagocytosis ； apoptosis ； Transglutaminase2

Abstract │ Reference (79) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Serologically active clinically quiescent (SACQ) patients was 8-12% of the total SLE population. SACQ is controversial in true inactive lupus or not. Impaired phagocytosis and clearance of apoptotic cells have been found in active lupus patients. However, this phenomenon is still controversial in SACQ lupus patients. 
Eighteen SACQ lupus patients were enrolled in this study. Eighteen age-matched healthy females served as controls. Macrophage phagocytosis of apoptotic cells was assessed in vitro using the serum-free phagocytosis assay with minor modifications. Adherent human monocyte-derived macrophages were used in this study. Compared to healthy controls, clinically inactive patients with SLE had significantly decreased phagocytosis ratio. 
SACQ SLE patients significantly impaired phagocytosis and clearance of apoptotic cells were still observed. Such phenomena might at least in part amplify further disease progression, also more enhanced the importance of intrinsic factor.
	The mechanism of autoimmune disease remains unclear. It has been thought that balance of cell survival and death is the main reason of autoimmune disease. Several studies indicated that transglutaminase 2 plays a potential role in cell apoptosis.
The aim of this study was to investigate the mRNA expression of TG2 on peripheral blood mononuclear cells (PBMCs) in lupus patients and further to determine its association with clinical features. 
Thirty-eight female lupus patients were enrolled in this study. Thirty three age-matched healthy subjects served as controls. Total RNA was isolated from PBMCs of patients with SLE and healthy subjects. The first-strand cDNA for RT-PCR was synthesized from total RNA using the RT-PCR system. The cDNAs encoding human TG2 and GAPDH were amplified by RT-PCR using the appropriate primer pairs, respectively.
The mRNA expression of TG2 in SLE patients was significantly higher than healthy controls. However, there was no significant difference for mRNA expression of TG2 between different age, disease activity, disease duration or serology data.
In previous study, apopotic cell induced TG2 expression and enhanced phagocytosis. SLE patient impaired phagocysosis and increased apopotosis, such phenomenon may postulate apoptotic cell compensatively increased TG2 overexpression on PMBC in SLE. It can’t conclude the mechanism of impaired phagocytosis in SLE patients, which emphasized macrophage defect itself and needs further investigation.

Reference ( 79 ) 〈TOP〉

Alon, M., Yael, S., Igor F., Yoav, P., Orly, E, and Michal, S., (1997). Expression of GTP-dependent and GTP-independent Tissue-type Transglutaminase in Cytokine-treated Rat Brain Astrocytes. J.Bio Chem, 272(6) 3724–3732.
連結：
Akimov, S. S., and Belkin, A. M., (2001). Cell surface tissue transglutaminase is involved in adhesion and migration of monocytic cells on fibronectin. Blood, 98, 1567-1576.
連結：
Bayardo, M., Punzi, F., Bondar, C., Chopita, N., and Chirdo, F., (2012). Transglutaminase 2 expression is enhanced synergistically by interferon-γ and tumour necrosis factor-α in human small intestine. Clinical & Experimental Immunology, 168(1):95-104.
連結：
Bengtsson, A. A., Sturfelt, G., Gullstrand, B., and Truedsson, L., (2004). Induction of apoptosis in monocytes and lymphocytes by serum from patients with systemic lupus erythematosus - an additional mechanism to increased autoantigen load? Clin Exp Immunol, 135, 535-543.
連結：
Bengtssona, A. A., Gullstrandb, B., Truedssonb, L., and Sturfelta, G., (2008). SLE serum induces classical caspase-dependent apoptosis independent of death receptors. Clin Immun, 126(1):57-66.
連結：

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：