3CL蛋白酶對於A549肺癌細胞顆粒球巨噬細胞聚落刺激因子生成的影響

廖憲華

doi:10.6834/CSMU.2012.00039

3.238.24.209，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

Health & Medical Care > Medical Sciences
College of Medicine > Graduate Institute of Medicine

Bibliography Management Tool

Related Links

Report an Issue

Purchase Single Articles

Download PDF

3CL蛋白酶對於A549肺癌細胞顆粒球巨噬細胞聚落刺激因子生成的影響

Effect of 3C-like proteinase on granulocyte-macrophage colony-stimulating factor gene expression in A549 human lung carcinoma cells

廖憲華 , Ph.D　　Advisor：蔡嘉哲;鄭森隆　　

繁體中文 DOI： 10.6834/CSMU.2012.00039

3CL蛋白酶顆粒球巨噬細胞聚落刺激因子； 3CL Protase ； GM-CSF

Abstract │ Reference (73) │ Altmetrics

Abstract 〈TOP〉

嚴重急性呼吸道症候群 (SARS) 是世界衛生組織於92年3月15日新公布的名稱，在這之前它被稱為非典型肺炎。感染特點為發生瀰漫性肺炎及呼吸衰竭，因為它比過去所知病毒、細菌引起的非典型肺炎病情更為嚴重，因此取名為嚴重急性呼吸道症候群 (severe acute respiratory syndrome, SARS)。嚴重急性呼吸道症候群的主要症狀為發高燒（＞38℃）、咳嗽、呼吸急促或呼吸困難。可能伴隨其他症狀，包括：頭痛、肌肉僵直、食慾不振、倦怠、意識紊亂、皮疹及腹瀉，胸部X光檢查可發現肺部病變。嚴重急性呼吸道症候群最嚴重時會出現瀰漫性肺炎，氧氣交換下降，導致肺部缺氧，所以病人會呼吸困難、缺氧，甚至導致死亡。
致病因子（Infectious agent）為在92年4月16日世界衛生組織正式宣布，SARS的致病原為新發現的冠狀病毒，並被正式命名為「SARS病毒」 (SARS- CoV)。(CDC Taiwan)
SARS病毒 (SARS- CoV) 的3C-like 蛋白酶對於病毒多胜肽前驅物質具有重要的影響，並且和病毒的突變有關聯。但是到目前為止，3C-like 蛋白酶在宿主的細胞中所扮演的角色和功能卻不是很明確的被清楚理解。本篇研究在於探討和研究3C-like蛋白酶對於基因轉染細胞的細胞激素 (cytokines) 的分泌的影響和其扮演的角色。

結果Results
從免疫螢光顯微鏡的結果來看，用c-myc 標示 (tagged) 的 3C-like 蛋白酶可以在被轉染的A549 細胞的細胞質和細胞核中定位發現出來。而不論是用反轉錄聚合酶鏈式反應 (RT-PCR) 分析或是酵素連結免疫分析法 (ELISA) 分析法，顆粒球巨噬細胞聚落刺激因子granulocyte-macrophage colony-stimulating factor (GM-CSF) 在 3C-like 蛋白酶轉染的細胞中的expression 顯著地下降，但對於其他的細胞激素 (cytokines) 例如: IL-1β, IL-6, IL-8, IL12p40, TNF-α, and TGF-β 卻沒有明顯改變。 此外，在和EGFP 轉染的細胞作比較時，在 3C-like 蛋白酶轉染的A549細胞中的NF-kB 的protein levels 較低。

結論Conclusion
經由本篇研究可推測3C-like 蛋白酶可以在被轉染的A549 細胞中，經由對NF-kB production 的down-regulation ，抑制GM-CSF的濃度表現量(expression)。

Parallel Abstract 〈TOP〉

Severe Acute Respiratory Syndrome (SARS) is a severe respiratory illness caused by a novel virus, the SARS coronavirus  (SARS-CoV) . 3C-like protease (3CLpro) of SARS-CoV plays a role in processing viral polypeptide precursors and is responsible of viral maturation. However, the function of 3CLpro in host cells remains unknown. This study investigated how the 3CLpro affected the secretion of cytokines in the gene-transfected cells.
Results
From immunofluorescence microscopy, the localization of c-myc tagged 3CLpro  was detected both in the cytoplasm and nucleus of transfected A549 cells. Expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) was significantly decreased in 3CLpro-transfected cells by both RT-PCR and ELISA, but without changes in other cytokines, i.e., IL-1β, IL-6, IL-8, IL12p40, TNF-α, and TGF-β. Furthermore, the protein levels of NF-kB decreased in 3CLpro-transfected A549 cells when compared to EGFP transfected cells.
Conclusions
Our results suggest that the 3CLpro  may suppress expression of GM-CSF in transfected A549 cells through down-regulation of NF-kB production.

Reference ( 73 ) 〈TOP〉

2. Rota PA, Oberste MS, Monroe SS, Nix WA, Campagnoli R, Icenogle JP, Penaranda S, Bankamp B, Maher K, Chen MH, et al.: Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 2003, 300:1394-9.
連結：
3. Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, Tong S, Urbani C, Comer JA, Lim W, et al.: A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med 2003, 348:1953-66.
連結：
5. Graham RL, Sparks JS, Eckerle LD, Sims AC, Denison MR: SARS coronavirus replicase proteins in pathogenesis. Virus Res 2008, 133:88-100
連結：
6. Prentice E, McAuliffe J, Lu X, Subbarao K, Denison MR: Identification and characterization of severe acute respiratory syndrome coronavirus replicase proteins.
連結：
J Virol 2004, 78:9977-86.
連結：

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：