斑馬魚雙氫雙烴基去氫酶在胚胎發生過程當中之表現

劉兆坤

doi:10.6834/CSMU.2006.00051

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斑馬魚雙氫雙烴基去氫酶在胚胎發生過程當中之表現

Expression of Dimeric Dihydrodiol Dehydrogenase of Danio rerio during embryo devolopement

劉兆坤 , Masters　　Advisor：劉德中　　

繁體中文 DOI： 10.6834/CSMU.2006.00051

NCBI、雙氫雙烴基去氫酶、原位雜交； National Center for Biotechnology Information ； Dimeric Dihydrodiol Dihydrogenase ； whole mount in situ hybridization.

Abstract │ Reference (24) │ Altmetrics

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In this study, the search tool BLAST (Basic Local Alignment Search Tool), based on NCBI (National Center for Biotechnology Information) was used against nucleotide and protein databases. The analysis found a Dimeric Dihydrodiol Dihydrogenase (dimeric DD) of zebrafish (NCBI accession number BC081393/ AAH81393) and human (NCBI accession number NM_014475/NP_055290). The dimeric DD cDNA of zebrafish and human were successfully amplified with a continuous open reading frame of 1005 bps and 1005 bps encoding a protein of 334 and 334 amino acids with a calculated molecular mass of 36.740 kDa and 36.740  kDa respectively.
    Zebrafish dimeric DD was cloned with a pGEM-T vector. To design a probe, which cdna of a antisense with DIG-UTP. Then, Zebrafish embryo were collected 24 hpf (hpf: hours post ertilization)、48 hpf、72 hpf and 96 hpf . Whole mount in situ hybridization analysis showed that dimeric DD is expressed in liver and pronephric ducts of 48 hpf、72 hpf and 96 hpf of Zebrafish embryo, not in 24 hpf of Zebrafish embryo. Therefore, this novel Dimeric Dihydrodiol Dihydrogenase is expressed in liver and pronephric ducts of larval Zebrafish not in more early stage.

Reference ( 24 ) 〈TOP〉

馬千茹(2005)。選殖及表現新穎之人類和斑馬魚之雙氫雙烴基去氫酶。中山醫學大學營養科學研究所碩士論文。
連結：
Aoki S,Ishikura S, Asada Y, Usami N , and Hara A.(2001) Identity of dimeric dihydrodiol dehydrogenase as NADP(+)-dependent D-xylose dehydrogenase in pig liver. Chem Biol Interact. 130-132, 775-84.
連結：
Arimitsu E, Aoki S, Ishikura S, Nakanishi K, Matsuura K, and Hara A. (1999) Cloning and sequencing of the cDNA species for mammalian dimeric dihydrodiol dehydrogenase. Biochem J. 342,721-8.
連結：
Armstrong B, Hutchinson E, and Fletcher T. (2003) Cancer risk following exposure to polycyclic aromatic hydrocarbons (PAHs): a meta-Analysis. Research Report 068, HSE Books, Health and Safety Executive.
連結：
Chen Y J, Yuan C C, Chow K C, Wang P H, Lai C R, Yen M S, and Wang L S. (2005) Overexpression of dihydrodiol dehydrogenase is associated with cisplatin-based chemotherapy resistance in ovarian cancer patients. Gynecologic Oncology. 97, 110-117.
連結：

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