KCNQ4 開啟劑衍伸物對於 Kv7.4 鉀離子電流的影響

蘇玫瑄

3.237.105.210，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

Health & Medical Care > Medical Sciences
College of Medicine > Graduate Institute of Medicine

Bibliography Management Tool

Related Links

Report an Issue

Purchase Single Articles

Download PDF

KCNQ4 開啟劑衍伸物對於 Kv7.4 鉀離子電流的影響

Studies of the novel KCNQ4 opener analogues on Kv7.4 potassium currents.

蘇玫瑄 , Masters　　Advisor：林明忠　　

繁體中文

鉀離子通道；結構相似物；癲癇；電生理；全細胞電位箝定； HEK293t 細胞； KCNQ4 開啟劑； porassium channels ； structure analogues ； epilepsy ； electriphysiology ； whole cell patch clamp ； HEK293t cells ； KCNQ4 openers

Abstract │ Reference (49) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

K+ channels are the most widely distributed ion channels in mammals and play an important role in stabilizing membrane potential in neurons and muscle cells. Mutation of K+ channels may result in ataxia, epilepsy. In the present study, we mainly focus on the function of KCNQ4 (Kv7.4) protein. There are five members of the KCNQ family, which are reffered to as either KCNQ1-KCNQ5. KCNQ4 is related to auditory system. KCNQ4 is expressed in inner ears, skeletal muscle and cardiac muscle. We are already known that ML213 and retigabine are the KCNQ4 openers. Clinically, retigabine has been treated with epilepsy, pain and arrhythmia. To identify the common structure, we designed the novel Kv7.4 channel openers. We used human embryo kidney cells (Hek293) to express KCNQ4, and recorded K+ currents before and after treated with new analogues by whole cell patch clamp technique.The preliminary results revealed that the amplitude of KCNQ4 K+ currents are increased by treated with retigabine (10μΜ), ML213(10μΜ), #3070(10μΜ), #0335(10 μM), #1848(10 μM)  #0298(10μΜ), #5907 (10 μM), #6696 (10 μM), 5540 (10 μM) and #8692 (10 μM). Furthermore we can find the activation curve was shifted toward less negative potentials by the treatment with KCNQ4 opener analogues (Retigabine, ML213, #3070, #1848, #0298, #0335, #5907, #6696, #5540 and #8692). Among these compounds, the #5907 and #5540 showed the most effective on KCNQ4 current. There are no effects on KCNQ4 with #3839(10μΜ), #1574(10 μM), #7294(10μΜ), #0321(10μΜ), #0331(10μΜ) and #5002(10μΜ). However the treatment with #0342 (10μΜ) causes the current amplitude decrease significantly. In conclusion, the KCNQ4 opener analogue, #5907 and #5540 showed more significant effect than the other nine compounds. Maybe we can further investigate the compound in the future.

Reference ( 49 ) 〈TOP〉

Afeli SA, Malysz J, Petkov GV. Molecular expression and pharmacological evidence for a functional role of kv7 channel subtypes in Guinea pig urinary bladder smooth muscle. PLoS ONE. 2013; 8: e75875.
連結：
Alisher M. Kariev and Michael E. Green. Voltage Gated Ion Channel Function: Gating, Conduction, and the Role of Water and Protons. Molecular Sciences. 2012; 13: 1680-1709.
連結：
Angeles M., Daniel G. N., Silvia M. H., Ainhoa E., Gracia A., Nieves S., Ignacio del C., Alvaro V., Felipe M., Luis B., Miguel A. M. P. A novel KCNQ4 pore-region mutation (p.G296S) causes deafness by impairing cell-surface channel expression. Hum Genet. 2008; 123: 41-53.
連結：
Bean BP. The action potential in mammalian central neurons. Nat Rev Neurosci.2007;8:451–465.
連結：
Brown D. A. and Adams P. R. Muscarinic suppression of a novel voltage-sensitive K+ current in a vertebrate neuron. Nature. 1980; 283:673-676.
連結：

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：