- 學位論文
洛神花多酚及花青素萃取物抗發炎作用之研究
Study of Hibiscus Sabdariffa Linnaeus polyphenols and anthocyanins extracts on anti-inflammation
摘要
華人社會中，洛神花一直被認為是飲食生活中很重要飲品，先前研究已證實其抗氧化效果。本研究主要以RAW264.7探討洛神花多酚、花青素對脂多醣體lipopolysaccharide (LPS)所誘發巨噬細胞的發炎媒介物質Nitrite、PGE2生成的影響，再以動物實驗模式，探討對大白鼠急性肝臟發炎的影響。在本研究結果發現洛神花多酚、花青素(0.01-0.5 mg/ml)可抑制黃嘌呤氧化酶活性(94.6%及10.5%)同時又能降低LPS活化的RAW264.7細胞nitrite 與PGE2的分泌。在動物實驗上，先予以管餵HPE(10, 20, 40 mg/kg)、HA(20, 40, 60 mg/kg)五天，並再LPS注射後六小時分析血清中測定肝功能的指標的酵素AST、ALT含量明顯的下降，且可降低肝臟脂質過氧化程度及增加過氧化氫酶 (catalase)、麩氨基硫 (GSH)等抗氧化性酵素；在肝臟病理組織中可以發現HPE、HA處理，可以減緩由LPS引起的肝臟組織發炎細胞浸潤程度。除此之外，也發現可以降低LPS誘導的COX-2 ， p-JNK and p-ERK的增加。在細胞實驗及在動物模式中發現，洛神花多酚、花青素具有降低LPS所誘發的發炎之能力，對於發炎相關疾病的影響或減緩發炎反應，值得進一步研究。
並列摘要
In Taiwan, Hibiscus Sabdariffa Linnaeus (HS), a well-known beverage, was demonstrated to represent antioxidant effect in previous studies. To further exploit the extracts, polyphenols and anthrocynins from HS (HPE and HA), we investigated the anti-inflammatory of effect of target sample in RAW264.7 cell on inflammatory mediators secretions (Nitrite and PGE2). Sequentially, animal model study was also preformed to confirm the anti-inflammatory effect of HPE and HA on lipopolysaccharide (LPS)-induced acute hepatic inflammation. The results showed that HPE and HA (0.01-0.5mg/ml) reduced the xanthine oxidase activity to 94.6% and 10.5% in the highest concentration in vitro, and also decreased the nitrite and PGE2 secretion in LPS-activated cells. The in vivo assay showed that pretreatment of HPE (10-40 mg/kg) or HA (20-60 mg/kg) for 5 days significantly decreased the levels of AST and ALT induced by LPS. Therefore also evaluate the antioxidant enzyme or antioxidant molecule, such as catalase and glutathione (GSH) and decrease the level of lipid peroxidation in liver. In histopathological evaluation of rat livers, it revealed that HPE and HA reduced the incidence of liver lesion such as inflammatory cell and infiltration induced by LPS. Furthermore, we also found that pretreated with HPE and HA decreased the hepatic protein expressions of COX-2, p-JNK and p-ERK induced by LPS in rats. These results showed that HPE and HA represented anti-inflammatory potential in vitro and in vivo; and they could be developed to nature, non-toxic anti-inflammatory materials.