研究在不同生長環境下結腸癌細胞對抗癌藥物之敏感性

李建緯

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研究在不同生長環境下結腸癌細胞對抗癌藥物之敏感性

Study of the anticancer drug sensitivity of colon cancer cell in the different growing environments

李建緯 , Masters　　Advisor：曾淑玲　　

繁體中文

大腸直腸癌；葡萄糖；癌幹細胞；抗藥性； Colorectal cancer ； glucose ； cancer stem cells ； drug resistance

Abstract │ Reference (38) │ Altmetrics

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In the UK and the USA, cancer is the second cause of death for the top ten, second only to cardiovascular disease. In Taiwan, one of the top ten causes of death is malignancy, in which colorectal cancer is one of the common malignancies. The incidence of colorectal cancer ranks first among men, women are second only to breast cancer. In recent years, morbidity and mortality have gradually increased, so for colorectal cancer prevention and treatment needs more attention. In the past few decades, although many efforts done in surgery, radiation therapy and basic research, but its five-year survival rate still not much improvement, and there have been many literature suggests that environment, eating habits are closely related to the incidence of colorectal cancer, therefore, if it can't effectively prevent colorectal cancer, need to first increase its survival rate and cure rate, to find a more efficient treatment of colorectal cancer, which has become an important issue in today's society. From the existing anti-cancer drugs combined with changes in cancer cell growth environment aspects, hoping to enhance the anticancer drugs sensitivity of cancer cells that enhance the cure rate of colorectal cancer. 
    Existing literature that high glucose promotes cancer cells proliferation, and make anti-cancer drug sensitivity decreased, but in colorectal cancer cells don't have a detailed mechanism to explore. Therefore, to understand the colorectal cancer cell lines SW480, SW620 in different concentrations of glucose environment, whether impact to the drug sensitivity, So use the 5-FU (Fluorouracil) that is the current mainly treatment of colorectal cancer, dosing treatment to observe the cell survival rate, MTT assay results show colorectal cancer cells grown at low concentrations of glucose have higher drug sensitivity. Next, using the flow cytometry to observe the expression of colorectal cancer cells in different glucose concentration environment, whether the cell cycle process is different. The results showed that under the stimulation of drugs, high glucose increase the S phase. On the other hand, there are many literatures that cancer stem cells have a significant effect on the drug resistance. Therefore, to understand whether different concentrations of glucose will increase the activity of cancer stem cells, so use sphere culture to observe the number of spherical cells and diameter size, the results showed that high concentrations of glucose contributes to cancer stem cell growth and activity.
    Based on the above results will be known, different concentrations of glucose have a certain influence on colorectal cancer cell drug resistance and cancer stem cells, control glucose concentration that will provide a effect on the treatment strategy for colorectal cancer patients, let healing ability and effectiveness have more improvement for colorectal cancer patients.

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Birsoy, K., Possemato, R., Lorbeer, F. K., Bayraktar, E. C., Thiru, P., Yucel, B., . . . Sabatini, D. M. (2014). Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides. Nature, 508(7494),108-112.
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連結：
Cb Penny, B. L. M. (2015). Cd133/Epcam Cancer Stem Cell Markers of Tumour Stage in Colorectal Cancer Cells. Journal of Tissue Science & Engineering, 06(01).
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Damdinsuren B, Nagano H, Kondo M, Natsag J, Hanada H, Nakamura M, Wada H, Kato H, Marubashi S, Miyamoto A. et al. TGF-beta1-induced cell growth arrest and partial differentiation is related to the suppression of Id1 in human hepatoma cells. Oncol Rep. 2006;15(2):401–408.
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