檸檬酸萃取文旦柚種子外殼多醣理化性質之探討

蔡銘澤

doi:10.6845/NCHU.2015.00136

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檸檬酸萃取文旦柚種子外殼多醣理化性質之探討

Studies on the physicochemical properties of polysaccharide from the outer hull of Citrus grandis seed with citric acid

蔡銘澤 , Masters　　Advisor：賴麗旭　　

繁體中文 DOI： 10.6845/NCHU.2015.00136

反應曲面法；黏質多醣；理化性質；酸萃取；柑橘類；植物廢棄物； response surface methodology ； mucilage polysaccharide ； physicalchemical properties ； acid-extracted ； citrus ； plant waste meterial

Abstract │ Reference (121) │ Times Cited (1) │ Altmetrics

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In this study, the effect of extraction variables, including citric acid concentration (0.25, 0.5, and 0.75 M), temperature (70, 80, and 90℃) and time (2, 4, and 6 hour) on the physicochemical properties of the mucilage extract from the seed hull of Citrus grandis Osbeck were investigated, and the optimal extraction conditions was developed by using response surface methodology with Box-Behnken design (BBD).  The results showed that citric acid residue in the mucilage increased with increasing concentration of citric acid, possibly due to the co-precipitation with polysaccharide fraction during polysaccharide precipitation step by using ethanol. Nevertheless, the protein content in mucilage was generally low for all treatments. Monosaccharide composition analysis revealed that the uronic acid content in mucilage generally decreased with increasing citric acid concentration, extraction temperature and extraction time. Moreover, the neutral sugar content was significantly lower than the uronic acid content for all treatments. The yields of the mucilage were generally raised as the citric acid concentration, extraction temperature and time increased, accompanied with a decrease in molecular weight and intrinsic viscosity, possibly due to certain extent of degradation of glycosidic bonds occurred under harsh extraction condition, leading to lower hydrodynamic volume occupied by the polysaccharide. However, the surface activity of all treatments was generally better than some polysaccharides reported in the literatures. The analysis of variance implied that the regression models obtained from the response surface analysis were pertinent to predict the extraction yield, surface tension, intrinsic viscosity, molecular weight and uronic acid content estimated by Carbazole method. By targeting a lowest surface tension (or highest surface activity), the optimum extraction conditions were found to be under a citric acid concentration of 0.503 M, extraction temperature of 82.32℃, and extraction time of 4.06 hr. The polysaccharide extracted under the optimal condition showed a surface tension value of 46.27 mN/m, which was not significantly different (p>0.05) from the predicted values calculated from the response surface model.

Reference ( 121 ) 〈TOP〉

10. 朱聖哲(2006)：培養模式與條件對紅茶姑的菌種活性、抗氧化力及茶多酚之影響。碩士論文。國立中興大學食品暨應用生物科技學研究所。台中市。台灣。
連結：
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連結：
17. 林志翰(2009)：微影製程駐波效應之改善研究。碩士論文。國立中興大學機械工程學研究所。台中市。台灣。
連結：
20. 徐永鑫、葉連德、黃湞鈺(2010)：湯種燙麵溫度、熟成時間與添加比例對土司物化性質之影響。Journal of Hospitality and Home Economics(餐旅暨家政學刊)。7(3)。285-298
連結：
22. 梁惠媛(2010)：水萃及鹼萃山蘇葉黏質之理化特性。碩士論文。國立中興大學食品暨應用生物科技學研究所。台中市。台灣。
連結：

Times Cited (1) 〈TOP〉

許進豐(2017)。以超音波結合酵素輔助萃取柚子種籽黏質及其理化性質之探討。中興大學食品暨應用生物科技學系所學位論文。2017。1-130。

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