香杉芝菌絲體乙醇萃取物對癌細胞侵襲與轉移之影響

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香杉芝菌絲體乙醇萃取物對癌細胞侵襲與轉移之影響

Effect of ethanolic extracts from Antrodia salmonea mycelium on invasion and metastasis in cancer cell

吳宣慧 , Masters　　Advisor：毛正倫　　

繁體中文

香杉芝； SK-Hep1 細胞； A549 細胞；細胞週期；癌細胞轉移； Antrodia salmonea ； SK-Hep1 cell ； A549 cell ； cell cycle ； metastasis

Abstract │ Reference (62) │ Altmetrics

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Lung cancer and liver cancer are the first and second cause of cancer death in Taiwan. The main cause of death is cancer cell invasion and metastasis to other important organs and tissues. Antrodia salmonea (syn. Taiwanofungus salmoneus) is an endemic fungus in Taiwan. It is a new species of the genus Antrodia in 2004, that same with Antrodia cinnamomea. The present studies have demonstraed that the extracts of A. salmonea have anti-inflammatory, antioxidation and anti-cancer abilities in vitro and in vivo. Moreover, the A. salmonea mycelium extract and fermentation products can induce cancer cells apoptosis. However, there is little information reported that A.salmonea extract in invasion and metastasis of cancer cells. Therefore, the purpose of this study was to investigate the effects of 70% ethanolic extracts (ASE) from A.salmonea mycelium on viability, invasion and metastasis of human lung adenocarcinoma cells (A549) and human hepatoma cells (SK-Hep1).
The results showed that the cell viability of SK-Hep1 was suppressed dose-dependently. The IC50 were 169.56g/mL and 119.04g/mL when cell were treated for 24 and 48 hours respectively. The cell viability of cell ASE on concentration 250g/mL was 85.98% and 74.84% in A549. In the cell cycle, SK-Hep1 was apoptosis via increasing the S phase and sub-G1 phases proportion. However, in A549 cells, G0/G1 phase was arrested on 24 hours and then S phase was arrested on 48 hours. There was no sub-G1 phase in A549. In wound healing assay, ASE could inhibit the migration ability of SK-Hep1 and A549. In gelatin zymography, the MMP-2 and MMP-9 enzymes of SK-hep1s and A549 were significantly inhibited in high-concentration of ASE after 24 hours, but the MMP-9 enzyme was mostly inactivated form. In western blot, the ASE did not show significantly effect in expression of MMP-2, MMP-9 and E-cadherin in SK-Hep1 cells, but inhibited the expression of E-cadherin in A549 cells. 
In conclusion, we demonstrated that 70% ethanolic extract of A. salmonea mycelium might suppress the proliferation and migration of SK-Hep1 and A549 by inducing G0/G1 phase or S phase arrest. It is significantly inhibited the invasion and metastasis in SK-Hep1 and A549 cells after treating ASE 24 hours.

Reference ( 62 ) 〈TOP〉

李佑軒。2015。幾種食藥用菇抗氧化、抗發炎活性及其抑制泡沫細胞形成能力。國立中興大學食品暨應用生物科技學系碩士論文。台中，台灣。
連結：
林雨潔。2015。香杉芝菌絲體及旗鼓太發酵產品之乙醇萃取物誘導癌細胞凋亡之作用。國立中興大學食品暨應用生物科技學系碩士論文。台中，台灣。
連結：
林蘭閔。2016。香杉芝菌絲體化學成分分析之抗氧化、抗發炎、與抗癌活性。國立中興大學食品暨應用生物科技學系碩士論文。台中，台灣。
連結：
張景明、張金堅。2014。談癌症治療的抗藥性。台灣醫界。57(3)。
連結：
謝昀蓉。2011。香杉芝菌絲體萃取物單獨及合併抗癌藥物對人類肝癌細胞豬凋亡之影響。國立中興大學食品暨應用生物科技學系碩士論文。台中，台灣。
連結：

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