探討顆粒性厭氧產氫反應槽中各微生物組成關係對產氫效能之影響

官荻偉

doi:10.6845/NCHU.2007.00503

54.236.62.49，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

College of Engineering > Department of Environmental Engineering
Engineering > Civil Engineering & Architecture Engineering

Bibliography Management Tool

Related Links

Report an Issue

Purchase Single Articles

Download PDF

探討顆粒性厭氧產氫反應槽中各微生物組成關係對產氫效能之影響

Explore the Relationship Between Microbial Community and Hydrogen Production in a Granule-Forming Anaerobic Fermentation Reaction

官荻偉 , Masters　　Advisor：洪俊雄　　

繁體中文 DOI： 10.6845/NCHU.2007.00503

厭氧生物醱酵產氫； Streptococcus sp. ； Klebsiella sp. ；顆粒污泥； Dark fermentation ； Streptococcus sp. ； Klebsiella sp. ； Granule activated sludge

Abstract │ Reference (166) │ Times Cited (7) │ Altmetrics

Abstract 〈TOP〉

厭氧生物醱酵產氫槽已被證實具有良好的產氫能力，此系統通常操作在常溫、常壓與中性pH條件下，不僅操作容易且不需投入大量的成本；厭氧生物醱酵產氫系統之產氫效能和系統中參與醱酵產氫的微生物族群，有著密切的關係，其中絕對厭氧菌Clostridium sp.被認定為厭氧醱酵菌中最具產氫潛力之菌種；本實驗室早期針對逢甲大學「生物氫能研究團隊」所架設之生物醱酵產氫槽進行菌相分析，結果顯示不同操作條件下，微生物菌相皆存在Clostridium sp.、Streptococcus sp.及Klebsiella sp.，當反應槽操作在低水力停留時間下，系統中僅剩C. pasteurianum及Streptococcus sp.，且反應槽操作在此條件下，具最佳的產氫速率，且同時可觀察到顆粒污泥的生成。本研究則欲釐清不同菌屬存在於產氫反應槽中對產氫效能之影響。
    結果指出C. pasteurianum最適生長溫度為37℃及初始pH值為7.0條件下，且在低碳源濃度下可得最大的氫氣產率為1.07 mol H2/mol glucose。K. oxytoca測試結果則顯示，其氫氣產量明顯低於C. pasteurianum，且此菌株在厭/好氧環境下皆能有效利用氧氣，因此可得知Klebsiella sp.存在於生物產氫反應槽中，能有效的消耗系統中的氧氣，使環境達到更厭氧的狀態以利絕對厭氧產氫菌的生長。菌株Strep. lutetieusis則在厭氧環境下具有分泌胞外多醣的能力，此類胞外多醣則具有黏稠等特性，因此推估Strep. lutetieusis所分泌的胞外多醣有助於細胞凝聚。透過Alcian blue染色結果指出，群聚的細胞中有胞外多醣的存在，且胞外多醣大多累積於污泥群聚的中心，因此可得知，胞外多醣生成與顆粒污泥形成的關係的確扮演著重要的角色。此外透過顆粒污泥切片觀察結果，亦可得知顆粒污泥的組成主要是以Clostridium sp.及Streptococcus sp.為主。
    綜合以上的結果可得知，生物產氫反應槽中主要的優勢菌種仍是以Clostridium sp.為主，Klebsiella sp.則可以消耗反應槽中的氧氣使環境達到更厭氧狀態，反應槽中常見的非產氫菌Streptococcus sp.，則扮演者凝聚細胞形成顆粒污泥的角色，顆粒污泥的生成則可以減少氫氣反應槽操作在低水力停留時間條件下污泥洗出的現象。

Parallel Abstract 〈TOP〉

The potential of efficient anaerobic fermentation reactor in hydrogen-production system had been well-documented. Benefits of applying these so-called dark fermentation bioreactor includes high hydrogen-producing capacity, high conversion efficiency, less energy intensive, less reactor volume needed and possible wastes reused. Microbial community structure analysis on sludge collected from our previous study showed that C. pasteurianum, Klebsiella sp. and Streptococcus sp. were the major microorganisms in the anaerobic fermentation reactors operated under differential hydraulic retention time. The best hydrogen producing efficiency occurred at low hydraulic retention time when a large amount of granules were self-formed and the predominated bacteria in these conditions were C. pasteurianum and Streptococcus sp. Therefore, the aim of this study is to understand the relationship between these microorganisms and their hydrogen-producing ability in a granule forming anaerobic fermentation reactor. 
    The best hydrogen yield and hydrogen production rate of by a pure culture of C. pasteurianum were observed at 37℃ and at an initial pH of 7.0. K. oxytoca could use oxygen as electron acceptor for growth, its further consumption of oxygen would lead to an anaerobic environment suitable for growth of strict anaerobic C. pasteurianum. Dominate bacterial groups existed in the granule activated sludge was proved to be Clostridium sp. and Streptococcus sp. by the using the Fluorescent in situ Hybridization technique (FISH). Result of Alcian Blue Stain shows that most of EPS existed in the center of aggregated bacterial granular and based on the pure culture experiments, this EPS complex was found to be produced by Strep. lutetieusis . Therefore, there is no doubt that these Streptococcus-produced EPS complexes played an important role in the forming of granule activated sludge. 
    From all of the data collected in this research, it can be concluded that Clostridium sp. is without a doubt the predominated hydrogen producing bacteria in anaerobic fermentation reactor. The existence of Klebsiella sp. would consume oxygen in this system and Streptococcus sp. was found to be in the position of helping bacteria aggregate and forming granule activated sludge, which can avoid being washout when the reactor was operated at low HRT.

Reference ( 166 ) 〈TOP〉

鄭幸雄, 李學霖, 趙禹杰及黃良銘 (2005) 利用澱粉及蛋白腖複合基質進行厭氧生物產氫程序. 第三十屆廢水處理技術研討會論文集. 第14頁, 桃園.
連結：
白明德 (1998) 厭氧生物產氫機制與程序操作策略之研究. 國立成功大學環境工程學系. 碩士論文,　台南.
連結：
林明正 (2000) CSTR厭氧產氫反應槽之啟動及操作. 逢甲大學土木及水利工程學系. 碩士論文, 台中.
連結：
鄭如琇 (2006) 以微生物組成探討厭氧醱酵系統之產氫效能. 國立中興大學環境工程學系. 碩士論文, 台中.
連結：
羅泳勝 (2005) 以反應曲面實驗設計法探討本土厭氧產氫菌Clostridium butyricum CGS2之最佳發酵產氫條件. 國立成功大學化學工程學系. 碩士論文,台南.
連結：

Times Cited (7) 〈TOP〉

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：