以PCR方法檢測芝麻油與花生油

楊亮渝

doi:10.6845/NCHU.2015.00634

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以PCR方法檢測芝麻油與花生油

Detection of sesame and peanut in oil by PCR method

楊亮渝 , Masters　　Advisor：蔡碩文　　Co-advisor ：蔣育錚

繁體中文 DOI： 10.6845/NCHU.2015.00634

芝麻油；花生油； PCR ； sesame oil ； peanut oil ； PCR

Abstract │ Reference (13) │ Altmetrics

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In the oil Products, sesame oil and peanut oil are the important in catering food. In recent years, several commercial oil contaminated event, the traditional oil row spring up again. The experiment will test the use of PCR to detect the DNA in the presence of oil or not, to find out if there are contained in the target of sesame and peanuts.
The samples were taken in place of the traditional press line oil and commercial oil. Standard: peanuts and sesame seeds. Target gene: peanut ITS1, sesame 2S albumin. Introduction group uses: PF / PR (peanuts, 181bp), SesF / SesR (sesame, 146bp). DNA extraction method with oil separately announcement PCR peanut and sesame oils qualitative detection, using 2% agar electrophoresis run, and finally the use of UV Fluorescence Imaging System and analyzed.
The results, regardless of flavor blending peanut oil, cold press oil extraction of oil or traditional, can be because the contents have been detected in the presence of peanuts, but there are shades of DNA fragments of the points. Sesame oil is obviously significant differences. The resulting DNA fragment shading sesame oil and peanut oil or the presence or absence inference might extraction methods, storage methods, extraction methods.
The purpose of this experiment is to use PCR method to detect sesame oil and peanut oil, with 100%  oil and mixed oil  to detect DNA fragments and DNA concentration is significantly high and low, to discuss commercial oil labeled correctly, and then apply the inspects of food oil products.

Reference ( 13 ) 〈TOP〉

劉廷航.王美琪.曾志正(2007)，芝麻種子油體和蛋白質體基礎研究及生物技術之應用。
連結：
林偉如.李敏雄.蘇南維(2011)，食用油混摻檢測方法之介紹。
連結：
S.Mustrop.C.Engdahl-Axelsson. Detection of celery(Apium graveolens), mustard(Sinapis alba, Brassica juncea, Brassica nigra) and sesame (Sesamum indicum) in food by real-time PCR. Eur Food Res Technol(2008) 226:771-778
連結：
A. Rayani.,F.D. Nayeri (2014), An improved method for extraction of high-quality total RNA from oil seeds.
連結：
F.Luber, A.Demmel, D.Herbert, A. Hosken, C. Hupfer, I. Huber, U.Busch, K., Engel (2014), Comparative assessment of DNA-based approaches for the quantification of food allergens. Food Chemistry 160(2014)104-111
連結：

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