以固定化海藻糖合成酶生產海藻糖之研究

程子鈞

doi:10.6845/NCHU.2011.00645

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以固定化海藻糖合成酶生產海藻糖之研究

Immobilization of recombinant trehalose synthase for the production of trehalose

程子鈞 , Masters　　Advisor：林松池　　

繁體中文 DOI： 10.6845/NCHU.2011.00645

海藻糖合成酶； trehalose synthase

Abstract │ Reference (60) │ Times Cited (1) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Trehalose is a kind of natural functional disaccharide.  In this study an integrated process following the concomitant purification and immobilization of recombinant trehalose synthase from Picrophilus torridus for the production of trehalose was established. Eupergit C was selected as a carrier in this work. The purified recombinant P. torridus TSase (PTTS) showed that the optimum pH and temperature of 6.0 and 50 °C, respectively, were not alternated upon immobilization. Nevertheless, the immobilized activity did exhibit the higher tolerance of temperature. A higher residual activity of 59.5% was observed for the immobilized enzyme under 70 oC whereas it was 4.1% for free enzyme.  The maximum conversion rate of maltose to trehalose by both free and immoblized enzyme was independent upon the substrate concentration under the optimum reaction conditions, in which the yield of approximately 60% for trehalose was obtained. The reusability of the immobilized enzyme in a batch reactor was also studied and we found that the immobilized enzyme maintained approximately 92.8% of residual activity after 24 cycles. While using the continuous packed-bed reactor, a maximum yield of 41.7% for trehalose has been reached from 100 mM maltose with a flow rate of 0.1 ml/min.

Reference ( 60 ) 〈TOP〉

1. Schiraldi, C.; Di Lernia, I.; De Rosa, M. Trehalose production: exploiting novel approaches. Trends Biotechnol. 2002, 20, 420-425.
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2. Elbein, A. D.; Pan, Y. T.; Pastuszak, I.; Carroll, D. New insights on trehalose: a multifunctional molecule. Glycobiology 2003, 13, 17-27.
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3. Brennan, P. J.; Nikaido, H. The envelope of mycobacteria. Annu.ReV. Biochem. 1995, 64, 29-63.
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4. Puech, V.; Chami, M.; Lemassu, A.; Laneelle, M. A.; Schiffler, B.; Gounon, P.; Bayan, N.; Benz, R.; Daffe, M. Structure of the cell envelope of corynebacteria: importance of the noncovalently bound lipids in the formation of the cell wall permeability barrier and fracture plane. Microbiology 2001, 147, 1365-1382.
連結：
5. Eastmond, P. J.; Graham, I. A. Trehalose metabolism:a regulatory role for trehalose-6-phosphate? Curr. Opin. Plant Biol. 2003, 6, 231-235.
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Times Cited (1) 〈TOP〉

葉文憲(2012)。利用再生纖維素膜共價鍵固定化開關蛋白作為開發生物感測器之研究。中興大學化學工程學系所學位論文。2012。1-85。

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