抗胡瓜嵌紋、胡瓜綠斑嵌紋與西瓜嵌紋病毒轉基因西瓜之研發

何琇銀

doi:10.6845/NCHU.2007.00664

3.232.133.141，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

College of Agriculture and Natural Resourses > Department of Agronomy
Bio-Agriculture > Agriculture

Bibliography Management Tool

Related Links

Report an Issue

Purchase Single Articles

Download PDF

抗胡瓜嵌紋、胡瓜綠斑嵌紋與西瓜嵌紋病毒轉基因西瓜之研發

Development of transgenic watermelon plants with multiple resistance to Cucumber mosaic-、Cucumber green mottle mosaic- and Watermelon mosaic viruses

何琇銀 , Masters　　Advisor：詹富智;古新梅　　

繁體中文 DOI： 10.6845/NCHU.2007.00664

轉基因西瓜；胡瓜嵌紋病毒；胡瓜綠斑嵌紋病毒；西瓜嵌紋病毒； transgenic watermelon ； Cucumber mosaic virus ； Cucumber green mottle mosaic virus ； Watermelon mosaic virus

Abstract │ Reference (89) │ Times Cited (2) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Watermelon is an important commercial crop in Taiwan and is prone to attack by several plant viruses.  Previous studies have shown that transgenic plants with multiple viral gene fragments linked to an universal silencer DNA can provide resistance to multiple virus via posttranscriptional gene silencing (PTGS).  In this study, similar approach was used to develop transgenic watermelon plants resistant to three different viruses via gene silencing.  Transformation constructs with selection marker gene and targer gene either in the same T-DNA or in different T-DNAs containing a silencer DNA from the partial N gene of Watermelon silver mottle virus (WSMoV) fused to the partial coat protein genes of Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV) and Watermelon mosaic virus (WMV) were generated and used to transform watermelon plant via Agrobacterium-mediated transformation.  A total of twenty-five trnasgenic lines were generated and confirmed by PCR amplification of selection marker gene.  Southern blot was used for analyzing the copy number of the target gene inserted the genome of each transgenic watermelon plant.   Transgenic watermelon plants were challenged with CMV, CGMMV, WMV or a mixture of these three viruses to evaluate the multiple virus resistance.  Among the twenty-five tested lines, three lines showed resistant to CGMMV and WMV.  Among these three resistant lines, two were also resistant to CMV and mixed inoculation of three viruses.  The result of northern blotting of transgenic watermelon plants showed a correlation between the resistant phenotype and low accumulation level of target gene RNA transcripts.  Our preliminary results indicate that transgenic watermelon carrying the fusion of different viral CP gene fragments can provide multiple virus resistance.  R1 seeds from the self-pollinated resistant R0 plants were obtained and will be used to evaluate the broad-spectrum resistance to CMV, CGMMV, WMV and WSMoV.

Reference ( 89 ) 〈TOP〉

沈百奎、鄧汀欽、余志儒、林俊義。2002。西瓜栽培管理。行政院農業委員會農業試驗所特刊第103號。台中。44pp。
連結：
林學詩。1995。西瓜及洋香瓜產業與研究。臺灣蔬菜產業改進研討會專集: 7-20。台中區農業改良場特刊第37號。台中。369pp。
連結：
林靜宜、詹富智。2005。無篩選標示基因轉基因植物之構築及其最新發展。植物病理學會刊14: 159-176。
連結：
Akashi, K., Morikawa, K., and Yokota, A. 2005. Agrobacterium-mediated transformation system for the drought and excess light stress-tolerant wild watermelon (Citrullus lanatus). Plant Biotechnol. 22: 13-18.
連結：
Baulcombe, D. C. 1996. RNA as a target and an initiator of post-transcriptional gene silencing in transgenic plants. Plant Mol. Biol. 32: 79-88.
連結：

Times Cited (2) 〈TOP〉

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：