金銀花萃取物免疫調節作用及其對前列腺癌PC-3與乳腺癌MCF-7細胞生長之影響

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金銀花萃取物免疫調節作用及其對前列腺癌PC-3與乳腺癌MCF-7細胞生長之影響

The immunomodulatory action of Lonicera japonica extracts and their effects on the growth of prostate cancer PC-3 and breast cancer MCF-7 cells

黃威齊 , Masters　　Advisor：林金源　　

繁體中文

抗發炎； CDK4基因； Cyclin D1基因；細胞激素；人類乳腺癌MCF-7細胞；人類前列腺癌PC-3細胞；金銀花；癌症免疫療法； anti-inflammation ； CDK4 gene ； Cyclin D1 gene ； cytokines ； human breast carcinoma MCF-7 cells ； human prostate carcinoma PC-3 cells ； Lonicera japonica ； tumor immunotherapy

Abstract │ Reference (78) │ Times Cited (2) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Lonicera japonica (LJ) is a traditional Chinese herbal medicine having the function of detoxication and fever cure. However, immunomodulatory effects of LJ remain unclear. To clarify the puzzle, LJ was consecutively extracted using three solvents from low polarity to high, including hexane, methanol and water, respectively. The extracts were first subjected to Th1/Th2 immunomodulatory study using mouse primary splenocytes and anti-inflammation evaluation using mouse primary peritoneal macrophages in vitro. Next, all of extracts were selected to treat human prostate cancer PC-3 cells and human breast cancer MCF-7 cells using direct addition or immunotherapy of primary peritoneal macrophages. Finally, the most potential extracts were isolated to explore possible mechanism against cancer cells. The results showed that hexane extracts (HELJ) and methanol extracts of Lonicera japonica (MELJ) rich in terpenoids and polyphenols, respectively, had potential to regulate Th1/Th2 immune responses toward Th1 immune balance and hot water extracts of Lonicera japonica (HWELJ) rich in polysaccarides toward to Th2 immune balance. HWELJ treatments in the presence of lipopolysaccharides (LPS) were found to have anti- inflammation potential and it was expected to have anti-cancer potential. To compare the anti-cancer effects of HWELJ and its fractions, HWELJ was further selected to purify with 75% alcohol and supernatant and precipitate of HWELJ was achieved. It was found that addition with HELJ and MELJ alone inhibited the proliferation of PC-3 and MCF-7 cells. Addition with HWELJ and its fractions alone  inhibited the proliferation of MCF-7 cells. Furthermore, treatments with conditioned media of HELJ and MELJ decreased the proliferation of PC-3 cells, especially HELJ conditioned medium. Hence, HELJ conditioned medium was selected to study its effect on the cell cycle of PC-3 cells. Consequently, addition with HELJ conditioned medium promoted cell cycle arrest in G1 phase by decreasing the CDK4 and Cyclin D1 mRNA expression in PC-3 cells.

Reference ( 78 ) 〈TOP〉

劉雅惠。2015。沉香萃取物之免疫調節功能及其對人類前列腺癌細胞和乳腺癌細
連結：
魏吟潔。2015。道手香水蒸餾精油吸入對正常及氣喘模式小鼠免疫調節之影響。
連結：
Balan, K., Qing, W. X., Wang, Y. Y., Liu, X. H., Palvannan, T., Wang, Y., Ma, F. Y., & Zhang, Y. (2016). Antidiabetic activity of silver nanoparticles from green synthesis using Lonicera japonica leaf extract. Rsc Advances, 6, 40162-40168.
連結：
Barnum, K. J., & O'Connell, M. J. (2014). Cell cycle regulation by checkpoints. Methods Mol Biol, 1170, 29-40.
連結：
Chaplin, D. D. (2010). Overview of the immune response. J Allergy Clin Immunol, 125, S3-23.
連結：

Times Cited (2) 〈TOP〉

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