Pectobacterium carotovorum subsp. carotovorum胞外分泌系統的結構蛋白與調控因子之關係探討

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Pectobacterium carotovorum subsp. carotovorum胞外分泌系統的結構蛋白與調控因子之關係探討

Studies of the cooperation mechanism between structure proteins and regulation factors in extracellular secretion system in Pectobacterium carotovorum subsp. carotovorum

陳坤弘 , Masters　　Advisor：莊敦堯　　

繁體中文

細菌素；第三類分泌系統； CRP調控因子； Pectobacterium ； Carocin ； Type III secretion system ； CRP regulation factor

Abstract │ Reference (76) │ Times Cited (1) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Pectobacterium carotovorum subsp. carotovorum is a Gram-negative, phytopara-sitic enterobacterium responsible for the soft-rot disease of the family Brassicaceae by producing extracellular pectinases. It causes greatly economic losses every year. So far, there is no efficient method, either chemical or otherwise, which has been found to con-trol this worldwide disease. Agrochemicals are generally used against this disease, but they cause the environmental pollutions.
Bacteriocins are antimicrobial and extracellular toxic proteins, which are produced by some bacteria and kill closely related bacteria. However, these proteinaceous toxins are highly specific and low environmental contaminations. They can be exploited as an-timicrobial agents and are efficiently preventing the pathogen that causes the economic damage.
The flagellum secretion system consisting of more than twenty different proteins is a rotary device for motility in liquid. From our previous studies, low-molecular-weight bacteriocin, Carocin, had already been confirmed and reported that it was exported to extracellular through the bacterial flagellum type III secretion system. In addition, from previous research, the regulation factors, DGC and CRP, play an important role in ex-pression mechanism of Carocin in Pcc.
In this study, we use RT-PCR method to analysis the mutants of strains, H-rif-8-6 and 3F-3. When the regulation factor, the crp gene, are interrupted, the flagellum stru-ture proteins can not be express normally. They are identified to be influenced each oth-ers’ normal expression. And then, we can know their cooperation mechanism.

Reference ( 76 ) 〈TOP〉

1. Malcolmson, J. F. A study of Erwinia isolates obtained from soft rots and black-leg of potatoes. T. Brit. Mycol. Soc. 1959, 42:261-269.
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2. Pérombelon, M. C. M. Potato diseases caused by soft rot erwinias: an overview of pathogenesis. Plant Pathol. 2002, 51:1-12.
連結：
3. Lund, B. M. Formation of Reducing Sugars from Sucrose by Erwinia Species. J. Gen. Microbiol. 1975, 88:367-371.
連結：
4. Jones, R. D. and Dowson, W. J. On the bacteria responsible for soft-rot in stored potatoes, and the reaction of the tuber to invasion by Bacterium carotovorum (Jones) Lehmann & Neumann. Ann. Appl. Biol. 1950, 37:563-569.
連結：
5. Phillips, J. A. and Kelman, A. Direct fluorescent antibody stain procedure applied to insect transmission of Erwinia carotovora. Phytopathology. 1982, 72:898-901.
連結：

Times Cited (1) 〈TOP〉

盧孝明(2017)。低分子量細菌素在Pectobacterium carotovorum subsp. carotovorum以類第三類分泌系統分泌至胞外之研究。中興大學化學系所學位論文。2017。1-57。

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