利用市售纖維素酶和半纖維素酶降解麥麩生成木寡糖與甘露寡糖

何慧琳

doi:10.6845/NCHU.2015.01353

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利用市售纖維素酶和半纖維素酶降解麥麩生成木寡糖與甘露寡糖

Use of commercial cellulase and hemicellulase on degrading wheat bran to produce xylo-oligosaccharide and mannooligosaccharide

何慧琳 , Masters　　Advisor：陳錦樹　　

繁體中文 DOI： 10.6845/NCHU.2015.01353

麥麩；纖維素酶；半纖維素酶；木寡糖；甘露寡糖； wheat bran ； cellulase ； hemicellulase ； xylooligosaccharide ； mannooligosaccharide

Abstract │ Reference (160) │ Altmetrics

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Wheat bran is a by-product of milling process during the production of flour. Wheat bran is composed of aleurone layer, testa and pericarp, and is rich in proteins, carbohydrates, fats, minerals, dietary fibers and other bioactive substances such as phenolic acids. Crude fiber in wheat bran includes cellulose, hemicellulose and lignin. After enzymatic or acidic treatment, the hemicellulosic component will be degraded into oligosaccharides. These oligosaccharides could provide multiple health effects, including selectively stimulating the growth of gastrointestinal probiotics and promotion of the host’s health. In this study, five grams of wheat bran were suspended in 50 mL water, followed by autoclaving. Hydrolysis reactions were subsequently performed by adding commercial cellulase and/or hemicellulose. The composition of oligosaccharides in the hydrolysates was analyzed using HPLC method. Effects of the mixing ratio (w/w) of two enzymes and hydrolysis time (0-12 h) on the kinds and amount of oligosaccharides were investigated. The results showed that oligosaccharides especially xylotriose (10.81 g/mL) and xylobiose (2.14 mg/mL) were detected in the hydrolysates right after the cellulase alone (0.5 g) was added. In addition, mannotriose at 0.64 mg/mL and mannotetraose at 0.63 mg/mL were detected, too. As for those using hemicellulase alone, not only xylotriose (8.68 mg/mL) and xylopentose (3.59 mg/mL) were found right after the addition of the enzyme, but also mannotriose at 0.27 mg/mL and mannotetraose at 0.18 mg/mL were detected. Finally, when combinations of cellulase and hemicellulase at ratio of 0.4 to 0.1 (w/w) were used, xylotriose at 13.78 mg/mL was detected immediately after the enzymes were added, followed by the appearance of xylobiose at 4.97 mg/mL after hydrolysis for 6 h. The above results indicated possible thermal destruction of wheat bran occurred during the autoclaving, both xylo- and manno-oligosaccharides were produced shortly whenever cellulase or hemicellulase alone or combinations of both enzymes were used, the hydrolysis reactions led eventually to production of hydrolysates with probiotic effect.

Reference ( 160 ) 〈TOP〉

行政院農業委員會農糧署。2013主要雜糧進口量值（年）。
連結：
characterization, bioactive properties, and applications. Comprehensive
連結：
Reviews in Food Science and Food Safety. 10:2-16.
連結：
Bastawade, K.B. (1992). Xylan structure, microbial xylanases, and their
連結：
mode of action.World Journal of Microbiology and Biotechnology.
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