小本山葡萄及羅氏鹽膚木蟲?成份之研究

Studies on the Constituents from Vitis thunbergii and the Galls of Rhus semialata

林煥哲(Lin Huan-Che)

指導教授 ： 徐鳳麟

臺北醫學大學/藥學院/藥學研究所/碩士(1998年)

摘要

在傳統製藥工業，得自植物或微生物之天然物，常為尋求新藥物或醫藥品〞前導化合物 〞最重要來源之一。近年國內外不同之研究群對於含有多量極性化合物之一些傳統草藥給予 特別之注意。自從 1980 年代鞣質化學領域之發展開始，由於新的分離技術及化學結構分析 方法之進展，逐漸轉變對極性化合物了解之深廣以及應用。同時，天然之多酚類等極性化合 物因而得以單一純化之物質快速呈現，造成了最近對其化學結構與藥理作用相關性之廣泛研 究。因此，本論文以小本山葡萄及羅氏鹽膚木之蟲?進行研究，探討其所含之極性化合物。 小本山葡萄為葡萄科植物，過去對於其極性物質研究甚少。其新鮮莖葉之 60 % 丙酮萃 取物，經各種管柱層析純化，共得六個化合物，其結構經光譜分析確認為 Caffeic acid (1 )、Quercetin-3-O-galactoside (2)、Quercitrin (3)、Rutin (4)、Narcissin (5) 及 Luteolin-7-O-glucoside (6)。其中Caffeic acid (1) 為主成份。 五倍子為某些植物蚜蟲螫刺鹽膚木(漆樹科)而形成者，極富於鞣質。羅氏鹽膚木為鹽膚 木之類緣植物，其之 60 % 丙酮萃取物，經由各種管柱層析，共得十七個化合物，其結構經 確認為 Shikimic acid (7)、Protocatechuic acid (8)、Gallic acid (9)、Methyl gall- ate (10)、Digallic acid (11) 、Methyl digallate (12)、1-O-galloyl-β-D-glucose ( 13)、1,2,3,4,6-penta-O-galloyl-β-D-glucose (14)、(+)-Catechin (15)、Eriodictyol (16)、Luteolin (17)、Quercetin (18)、(-)-Rhusflavone (19)、Agathisflavone (20)、 Amentoflavone (21)、Butein (22)、p-Hydroxyacetophenone (23)。其中Gallic acid (9) 為其主成份。

關鍵字

小本山葡萄 ； 羅氏鹽膚木 ； 蟲

並列摘要

Natural Products from plants and microorganisms traditionally have provided the pharmaceutical industry with one of its most important sources of " lead compounds " in the search for new drugs and medicines. In this context, particular attention has been given by various groups in the Far East, Europe, and America to those traditional herbal medicines rich polyphenols (vegetable tannins). In the 1960''s the advent of new methods of isolation and analysis gradually revolutionized the depth of scientific understanding and appreciation of this field; natural polyphenols become available as single pure entities, and this has made possible recent extensive studies of the relationship of structure to biological and pharmacological activity.Therefore, a chemical investigation of polar compounds in Vitis thunbergii S. et Z. and Rhus semialata Murr. var. roxburghii DC were performed. Vitis thunbergii S. et Z. ( Vitaceae ). In the past, their polar constituent s and related compounds are still remained to be investigated. The leaves were extract with 60 % aqueous acetone. By means of various column chromatographies, six compounds were isolated and purified. The structures of these compounds were elucidated as Caffeic acid (1), Quercetin-3-O-galactoside (2), Quercitrin (3), Rutin (4), Narcissin (5), Luteolin-7-O-glucoside (6). Among them, Caffeic acid ( 1) were the major components. Chinese galls are formed on Rhus chinensis Mill ( Fam. Anacardiaceae ) owing to the stings of certain plant lice. Galls are used as a source of tannic acid. Rhus semialata Murr. var. roxburghii DC., an allied plant of Rhus semialata Murr ., the galls were extract with 60 % aqueous acetone. By means of various column chromatographies, seventeen compounds were isolated and purified. The structures of these compounds were elucidated as Shikimic acid (7), Protocatechuic acid (8) , Gallic acid (9), Methyl gallate (10), Digallic acid (11) , Methyl digallate ( 12), 1-O-galloyl-β-D-glucose (13) , 1,2,3,4,6-penta-O-galloyl-β-D-glucose (14) , (+)-Catechin (15), Eriodictyol (16), Luteolin (17), Quercetin (18), (-)-Rhus- flavone (19), Agathisflavone (20), Amentoflavone (21), Butein (22) , p-Hydroxy- acetophenone (23). Among them, Gallic acid were the major components.

並列關鍵字

Vitis thunbergii ； Rhus semialata ； Galls