喜樹幼葉成分之研究

Studies on the constituents of the young leaves of Camptotheca acuminata

楊士平(Shih-Ping Yang)

指導教授 ： 李慶國

臺北醫學大學/藥學院/生藥學研究所/碩士(2009年)

摘要

喜樹 (Camptotheca acuminata Decaisne) 為紫樹科 (Nyssaceae) 喜樹屬植物，為原產於中國大陸的落葉性喬木，主要分布在四川、雲南跟廣西等溫暖潮濕地區。喜樹裡最著名的二次代謝物是為喜樹鹼(Camptothecin, CPT)，在1960年代被發現具有良好的抗腫瘤活性。
本研究利用喜樹幼葉部以乙醇浸泡萃取，浸泡液經減壓濃縮後，濃縮液經由水、乙酸乙酯以及正丁醇作分配萃取。取乙酸乙酯層及正丁醇層部份分別利用矽膠管柱層析、全自動色層分析儀作初步分離，之後利用薄層層析法、高效能液相層析法等方法，分離純化得到41個化合物，其中包括九個aliphatic類、一個monoterpenoid類、一個diterpenoid類、二個triterpenoid類、一個steroid類、一個pheophorbide類、六個aromatic類、七個flavonoid類、一個norsesquiterpenoid類、一個aliphatic glycoside類、一個secoiridoid glycoside類、二個quinoline類、三個quinic acid類、四個others類，其中化合物CA36為新化合物。

關鍵字

喜樹 ； 紫樹科 ； 喜樹鹼 ； 生物活性導引分離

並列摘要

Camptotheca acuminata Decaisne (Nyssaceae), a deciduous tree native to China, is widely distributed in relatively warm areas such as Sechwan, Yunnan, and Kwangsi. The secondary metabolites, Camptothecin (CPT) which was found to possess promising anti-tumor activity in the 1960s is isolated from C. acuminata.
In this study, the ethanol extract of young leaves of Camptotheca acuminata Decaisne was concentrated to give a residue which was partition with water, ethyl acetate, and butanol. The ethyl acetate layer and butanol layer were separated by column chromatography on silica gel, automated flash chromatography, thin layer chromatography, and high performance liquid chromatography to afford forty-one compounds. Including nine aliphatic compounds, one monoterpenoid compound, one diterpenoid compound, two triterpenoid compounds, one steroid compound, one pheophorbide compound, six aromatic compounds, seven flavonoid compounds, one norsesquiterpenoid compound, one aliphatic glycoside compound, one secoiridoid glycoside compound, two quinoline compounds, three quinic acid compounds, and four others compounds. Among them, CA36 is a new compound.

並列關鍵字

Camptotheca acuminata Decaisne ； Nyssaceae ； Camptothecin ； Bioactivity-directed fractionation

參考文獻

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