應用液相層析串聯式質譜技術於台灣產蕨類植物特定蕨素成分之分析研究

黃秋羽

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應用液相層析串聯式質譜技術於台灣產蕨類植物特定蕨素成分之分析研究

Applying LC-MS/MS to the Analysis of Pterosins in Pteridophytic Floras of Taiwan

黃秋羽 , Masters　　Advisor：徐鳳麟　　Co-advisor ：林哲輝

繁體中文

蕨素；液相層析串聯式質譜技術；正離子電灑法；多重離子裂解監控模式； pterosin ； chromatography-tandem mass spectrometry ； positive ion electrospray ionization ； multiple reaction monitoring

Abstract │ Reference (66) │ Altmetrics

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Abstract
   This study aimed to establish an analytical method for screening certain types of pterosins and studies the distribution of those in ferns. Pterosins are a large group of naturally occurring sesquiterpenes with indanone skeleton. Widely distributed in the Pteridophyte, having been found to have cytotoxicity and smooth muscle relaxant activity, there are still new findings related to pterosins in the ongoing pharmacological study. Here we introduced a newly developed method of applying LC-MS/MS to the analysis of pterosins in ferns and conducted a survey towards 25 species of ferns from Taiwan.
The optimized LC-MS/MS condition was as follows. Separation was carried out using a reverse phase C18 column under gradient elution. Mobile phase comprised a mixed solvent system of 0.25% formic acid-water-acetonitrile and was set at a flow rate of 0.5 mL/min with a split ratio of 1:1 into PDA and tandem mass spectrometer. The electrospray ionization source was operated in the positive mode. The mass parameters including cone voltage, capillary voltage, collision energy, ion source temperature, desolvation temperature and desolvation gas flow were subsequently optimized. Proton adduct ions of each analyte were fairly detectable under current analytical condition and were selected as parent ions for further daughter ion scan. Qualitative analysis was achieved by daughter ion analysis. The characteristic daughter ion mass spectra of pterosins were generated for library search. Quantitative analysis was accomplished by MRM (multiple reaction monitoring) analysis. Limits of detection using MRM analysis were defined as 9.97 ng/mL of pterosin A, 7.47 ng/mL of pterosin I, and 3.60 ng/mL of pterosin Z. R.S.D. of intraday and interday repeated analysis were within 7.52% and 11.95%, respectively.
The result shows that pterosins were detected in 10 species out of 25, mainly found in the families of Dennstaedtiaceae and Pteridaceae, mostly in the non-polar or less polar partition layer such as n-hexane, dichloromethane or ethyl acetate. Levels of pterosin A, pterosin I and pterosin Z detected in the 10 species of ferns ranged from 1.15 to 9615.4 μg/g, from 6.3 to 10845.5 μg/g and from 11.0 to 15480.2 μg/g, respectively. Amounts of pterosin A were found to be highest in P. aquilinum var. latiusculum while amounts of pterosin I and Z were highest in M. speluncae. Mass information of those signals with pterosin-like UV spectrum in the chromatographic system was recorded. A fast, sensitive and highly specific method coupling both a photodiode array (PDA) detector and tandem mass spectrometer was developed in this study.

Reference ( 66 ) 〈TOP〉

6. K. Yoshihira, M. Fukuoka, M. Kuroyannagi, S. Natori, M. Umeda, T. Morohoshi, M. Enomoto, M. Saito. 1978. Chemical and toxicological studies on bracken fern, Pteridium aquilinum var. latiusculum. I. Introduction, extraction and fractionation of constituents, and toxicological studies including carcinogenicity tests. Chem. Pharm. Bull. (Tokyo), 26; 8: 2346-64.
連結：
7. 賴國誌。2003。姬蕨抗癌成分之研究。台北醫學大學生藥學研究所碩士論文。台北。
連結：
8. H. Sheridan, N. Frankish, R. Farrell 1999. Smooth muscle relaxant activity of pterosin Z and related compounds. Planta Med., 65; 3: 271-272.
連結：
9. 林玉英。2007。巒大蕨活性成分之探討。台北醫學大學生藥學研究所碩士論文。台北。
連結：
11. K. Yamada, M. Ojika, H. Kigoshi. 2007. Ptaquiloside, the major toxin of bracken, and related terpene glycosides: chemistry, biology and ecology. Nat. Prod. Rep., 24; 4: 798-813.
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